Grunwald G B, Gilbert S F, Brewer K, Cleland L, Kawai M
Department of Anatomy, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
Histochemistry. 1990;94(5):545-53. doi: 10.1007/BF00272620.
Mab 113F4, a monoclonal antibody recognizing an antigen in the outer synaptic layer of the chick neural retina, also recognizes an antigen appearing in all three germ layers of the gastrulating chick embryo. However, as neurulation proceeds, the antigen is down-regulated in three distinct patterns. First, the antigen is lost specifically from those trunk ectodermal cells destined to form the neural plate and, later, the neural tube. It remains absent from any neural derivative until day 13 when it appears in the outer synaptic layer of the neural retina, coincident with synaptogenesis in this region. Second, the entirety of the head ectoderm loses this antigen as the head lifts off the blastoderm. This down-regulation is followed later by a similar loss of antigen expression in the trunk ectoderm. Third, expression in the mesoderm becomes limited to the lateral plate and extraembryonic epithelia. Endodermal derivatives continue to express the antigen throughout development. Antigen 113F4 is localized within the cytoplasm and is organized in a fibrillar pattern. The intracellular localization of this antigen and its characteristic spatio-temporal tissue distribution are consistent with the antigen being a cytokeratin or cytokeratin-related antigen. The changes in tissue distribution suggest a possible role in tissue modelling in response to inductive interactions during development.
单克隆抗体113F4可识别鸡神经视网膜外突触层中的一种抗原，它也能识别原肠胚形成期鸡胚所有三个胚层中出现的一种抗原。然而，随着神经胚形成过程的推进，该抗原以三种不同模式下调。首先，该抗原特异性地从那些注定要形成神经板以及随后神经管的躯干外胚层细胞中消失。在第13天之前，任何神经衍生物中都没有该抗原，直到第13天它出现在神经视网膜的外突触层，与该区域的突触发生同时出现。其次，随着头部从胚盘抬起，整个头部外胚层失去这种抗原。这种下调之后，躯干外胚层也会出现类似的抗原表达丧失。第三，中胚层中的表达局限于侧板和胚外上皮。内胚层衍生物在整个发育过程中持续表达该抗原。抗原113F4定位于细胞质内，并呈纤维状分布。这种抗原的细胞内定位及其特征性的时空组织分布与该抗原是一种细胞角蛋白或细胞角蛋白相关抗原相一致。组织分布的变化表明其在发育过程中对诱导相互作用做出反应的组织建模中可能发挥作用。
