Grunwald G B, Geller R L, Lilien J
J Cell Biol. 1980 Jun;85(3):766-76. doi: 10.1083/jcb.85.3.766.
In this paper we describe a kinetic assay for cell adhesion which measures the formation of cell clusters. Cluster formation is dependent on both calcium and protein synthesis, two parameters essential for the formation of histotypic aggregates. We also describe modifications of the stndard method for trypsinization of tissues which result in populations of single cells that appear to bear intact and functional cell surface adhesive systems. These modifications involve the use of chymotrypsin and the inclusion of calcium during enzyme digestion of tissues with trypsin and chymotrypsin. Using the cluster formation assay and the modified tissue dissociation techniques, we demonstrate the presence of two functionally distinct adhesive systems operating among embryonic chick neural retina cells. These two systems differ in proteolytic sensitivity, protection by calcium against proteolysis, dependence on calcium for function and morphogenetic potential. Cells possessing one of these intact adhesive systems are capable of extensive morphogenetic interactions in the absence of protein synthesis.
在本文中,我们描述了一种用于细胞黏附的动力学测定方法,该方法可测量细胞簇的形成。簇的形成依赖于钙和蛋白质合成,这是形成组织型聚集体所必需的两个参数。我们还描述了用于组织胰蛋白酶消化的标准方法的改进,这些改进产生了单细胞群体,这些单细胞似乎具有完整且功能正常的细胞表面黏附系统。这些改进包括使用胰凝乳蛋白酶以及在用胰蛋白酶和胰凝乳蛋白酶消化组织期间加入钙。使用簇形成测定法和改进的组织解离技术,我们证明了在胚胎鸡神经视网膜细胞中存在两种功能不同的黏附系统。这两种系统在蛋白水解敏感性、钙对蛋白水解的保护作用、功能对钙的依赖性以及形态发生潜力方面存在差异。拥有这些完整黏附系统之一的细胞在没有蛋白质合成的情况下能够进行广泛的形态发生相互作用。