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间皮素作为细胞学标本中间皮细胞标志物的应用。

Use of mesothelin as a marker for mesothelial cells in cytologic specimens.

作者信息

Kachali Cornelius, Eltoum Isam, Horton Debra, Chhieng David C

机构信息

Department of Pathology, University of Alabama, Birmingham, Alabama 35249-6823, USA.

出版信息

Semin Diagn Pathol. 2006 Feb;23(1):20-4. doi: 10.1053/j.semdp.2006.06.006.

DOI:10.1053/j.semdp.2006.06.006
PMID:17044192
Abstract

Immunocytochemistry is often employed for the distinction between mesothelial cells and adenocarcinoma. Mesothelin has recently been reported to be expressed in reactive mesothelial cells and epithelioid mesotheliomas. The objective of this study is to determine the utility of mesothelin as marker for mesothelial cells in cytologic preparations. Thirty cell blocks were retrieved from the archives and immunostained with monoclonal antibody directed against mesothelin and calretinin. Heat-induced epitope retrieval technique was employed, and the immunostaining was accomplished using an automated stainer. These tissue blocks were from 35 patients (17 females and 18 males) with a median age of 64 years. Nine were benign effusions, 11 mesotheliomas, and 18 metastatic adenocarcinomas. The presence of any immunoreactivity, irrespective of level of intensity or percentage of cells, was considered positive for mesothelin expression. Follow up included correlation with pathology materials obtained at surgery and review of medical records. Mesothelin staining was positive in 7/9 benign cases, 8/11 mesotheliomas, and 8/18 adenocarcinomas. The difference of mesothelin expression between mesothelial cells and adenocarcinoma was statistically significant. For calretinin, all cases, except 2 malignant mesotheliomas and 3 adenocarcinomas, showed positive staining with calretinin. As a marker for mesothelial cells, the sensitivity and specificity of mesothelin were 73% and 55%, respectively, and the sensitivity and specificity of calretinin were 95% and 86%, respectively. Therefore, mesothelin is not a sensitive or a specific marker for mesothelial cells in cytologic specimens when compared with calretinin.

摘要

免疫细胞化学常用于区分间皮细胞和腺癌。最近有报道称间皮素在反应性间皮细胞和上皮样间皮瘤中表达。本研究的目的是确定间皮素作为细胞学标本中间皮细胞标志物的效用。从档案中检索出30个细胞块,并用针对间皮素和钙结合蛋白的单克隆抗体进行免疫染色。采用热诱导抗原修复技术,并使用自动染色仪完成免疫染色。这些组织块来自35例患者(17名女性和18名男性),中位年龄为64岁。其中9例为良性积液,11例为间皮瘤,18例为转移性腺癌。无论强度水平或细胞百分比如何,任何免疫反应性的存在都被视为间皮素表达阳性。随访包括与手术时获得的病理材料进行对照以及查阅病历。间皮素染色在7/9例良性病例、8/11例间皮瘤和8/18例腺癌中呈阳性。间皮细胞和腺癌之间间皮素表达的差异具有统计学意义。对于钙结合蛋白,除2例恶性间皮瘤和3例腺癌外,所有病例均显示钙结合蛋白染色阳性。作为间皮细胞的标志物,间皮素的敏感性和特异性分别为73%和55%,钙结合蛋白的敏感性和特异性分别为95%和86%。因此,与钙结合蛋白相比,间皮素在细胞学标本中不是间皮细胞的敏感或特异标志物。

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