Barberis M C, Faleri M, Veronese S, Casadio C, Viale G
Emilio Bernardelli Medical Foundation, Paderno Dugnano, Milan, Italy.
Acta Cytol. 1997 Nov-Dec;41(6):1757-61. doi: 10.1159/000333181.
To document that a polyclonal antiserum to calretinin, a 29-kd calcium-binding protein, consistently decorates normal and tumor mesothelial cells in cytologic preparations.
Thirty-three archival cytologic specimens from eight patients with histologically confirmed malignant mesothelioma and 13 from patients with metastatic serous effusions were destained and then immunostained with anticalretinin antiserum. For investigation of cell suspensions, four pleural fluids were incubated with anticalretinin antiserum. After cytocentrifugation the specimens were stained in accordance with the alkaline phosphatase anti-alkaline phosphatase (APAAP) method. For electron microscopic examination the cell suspensions were then incubated with gold-labeled antirabbit antibody.
The diagnostic sensitivity of this new immunocytochemical approach reached 100% for the eight malignant mesotheliomas investigated. Only 3 of the 13 adenocarcinomas metastatic to the serous membranes included in this study were weakly reactive, accounting for 81% specificity. Binding of anticalretinin antiserum to living mesothelial cells was consistently documented in all four cases investigated.
Calretinin is a very useful marker for positive identification of normal and tumor mesothelial cells in serous effusions.
证实一种针对钙视网膜蛋白（一种29kd的钙结合蛋白）的多克隆抗血清在细胞学标本中能持续标记正常和肿瘤间皮细胞。
对8例经组织学确诊为恶性间皮瘤患者的33份存档细胞学标本以及13例转移性浆液性积液患者的标本进行脱色处理，然后用抗钙视网膜蛋白抗血清进行免疫染色。为研究细胞悬液，将4份胸水与抗钙视网膜蛋白抗血清孵育。经细胞离心后，标本按照碱性磷酸酶抗碱性磷酸酶（APAAP）法进行染色。为进行电子显微镜检查，随后将细胞悬液与金标记抗兔抗体孵育。
对于所研究的8例恶性间皮瘤，这种新的免疫细胞化学方法的诊断敏感性达到100%。本研究纳入的13例转移至浆膜的腺癌中只有3例呈弱阳性反应，特异性为81%。在所研究的所有4例病例中均证实抗钙视网膜蛋白抗血清与活间皮细胞有结合。
钙视网膜蛋白是浆液性积液中正常和肿瘤间皮细胞阳性识别的非常有用的标志物。
