Przyboś Ewa, Maciejewska Agnieszka, Skotarczak Bogumiła
Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016 Krakow, Poland.
Folia Biol (Krakow). 2006;54(1-2):37-42. doi: 10.3409/173491606777919157.
A fragment ofhistone H4 gene (160 bp long) was sequenced in the standard strains of P. primaurelia (DQ067620), P. biaurelia (DQ067621), P. tetraurelia (DQ067622), P. pentaurelia (DQ067623), P. septaurelia (DQ067624), P. octaurelia (DQ067625), P. decaurelia (DQ067626), P. undecaurelia (DQ067627), P. dodecaurelia (DQ067628), P. tredecaurelia (DQ067629), and P. quadecaurelia (DQ067630). The tree constructed according to the Kimura model presents two main species clusters, one comprising P. undecaurelia, P. octaurelia, P. septaurelia, and the second cluster with P. pentaurelia, P. tredecaurelia, P. quadecaurelia, P. tetraurelia, P. decaurelia, P. primaurelia, P. biaurelia. P. dodecaurelia was recovered as a separate branch. The tree constructed on the basis of the maximum likelihood method also presents two species clusters, one with P. undecaurelia, P. octaurelia, P. septaurelia, and the second with P. primaurelia, P. decaurelia, P. pentaurelia, P. tredecaurelia, P. quadecaurelia, P. tetraurelia. P. biaurelia forms a basal clade to the latter cluster, and P. dodecaurelia was recovered as a clearly distinct branch from the clusters.
对双小核草履虫（DQ067620）、双尾草履虫（DQ067621）、四尾草履虫（DQ067622）、五尾草履虫（DQ067623）、七尾草履虫（DQ067624）、八尾草履虫（DQ067625）、十尾草履虫（DQ067626）、十一尾草履虫（DQ067627）、十二尾草履虫（DQ067628）、十三尾草履虫（DQ067629）和十四尾草履虫（DQ067630）的标准菌株中的组蛋白H4基因片段（160个碱基对长）进行了测序。根据木村模型构建的树呈现出两个主要的物种簇，一个包含十一尾草履虫、八尾草履虫、七尾草履虫，第二个簇包含五尾草履虫、十三尾草履虫、十四尾草履虫、四尾草履虫、十尾草履虫、双小核草履虫、双尾草履虫。十二尾草履虫作为一个单独的分支被分离出来。基于最大似然法构建的树也呈现出两个物种簇，一个包含十一尾草履虫、八尾草履虫、七尾草履虫，另一个包含双小核草履虫、十尾草履虫、五尾草履虫、十三尾草履虫、十四尾草履虫、四尾草履虫。双尾草履虫形成了后一个簇的基部进化枝，十二尾草履虫作为一个与这些簇明显不同的分支被分离出来。
