Department of Ecology, University of Kaiserslautern, Erwin-Schrödinger-Strasse 14, D-67663, Kaiserslautern, Germany.
J Eukaryot Microbiol. 2012 Nov-Dec;59(6):548-63. doi: 10.1111/j.1550-7408.2012.00638.x. Epub 2012 Jul 24.
We redescribe Paramecium chlorelligerum, a forgotten species, which Kahl (Tierwelt Dtl., 1935, 30:651) briefly but precisely described in the addendum to his ciliate monographs as a Paramecium with symbiotic green algae. The redescription is based on classical morphological methods and the analysis of the small subunit (SSU) rDNA. Morphologically, P. chlorelligerum differs from P. (C.) bursaria, the second green species in the genus, by having a special swimming shape, the length of the caudal cilia, the size of the micronucleus, the size of the symbiotic algae, the contractile vacuoles (with collecting vesicles vs. collecting canals), and the number of excretory pores/contractile vacuole (1 vs. 2-3). The molecular investigations show that P. chlorelligerum forms a distinct branch distant from the P. (Chloroparamecium) bursaria clade. Thus, we classify P. chlorelligerum in a new subgenus: Paramecium (Viridoparamecium) chlorelligerum. The symbiotic alga belongs to the little-known genus Meyerella, as yet recorded only from the plankton of a North American lake.
我们重新描述了 Paramecium chlorelligerum，这是一种被遗忘的物种，Kahl（Tierwelt Dtl.，1935，30：651）在他的纤毛动物专论的附录中简要但准确地描述了它，是一种具有共生绿藻的草履虫。这次重新描述是基于经典的形态学方法和小亚基（SSU）rDNA 的分析。从形态学上看，P. chlorelligerum 与属中的第二种绿色物种 P.（C.）bursaria 不同，它具有特殊的游动形状、尾纤毛的长度、小核的大小、共生藻类的大小、伸缩泡（具收集囊 vs. 收集管）以及排泄孔/伸缩泡的数量（1 对 2-3）。分子研究表明，P. chlorelligerum 形成了一个与 P.（Chloroparamecium）bursaria 分支明显不同的分支。因此，我们将 P. chlorelligerum 分类到一个新的亚属：Paramecium（Viridoparamecium）chlorelligerum。共生藻类属于鲜为人知的 Meyerella 属，迄今为止仅从北美的一个湖泊的浮游生物中记录到。
