Lan Wen-Jun, Zheng Xiao-Xiang
Key Laboratory for Biomedical Engineering of Ministry of Education of China, Zhejiang University (Yuquan Campus), Hangzhou 310027, Zhejiang, China.
Biomed Environ Sci. 2006 Aug;19(4):249-53.
In order to investigate the pharmacological properties of Ginkgo biloba extract (GBE) on improving blood circulation, the regulating action of GBE and quercetin (a main flavonoid ingredient in GBE) on thrombomodulin (TM) expression and tissue-type plasminogen activator (t-PA) secretion was studied.
Using flow cytometer and gel image system respectively, we evaluated the TM expression and the t-PA secretion by human umbilical vein endothelial cells (HUVECs) in vitro.
The increase of TM expression on HUVECs surface was induced by GBE rather than quercetin in a dose- and time-dependent manner. Both GBE and quercetin increased the t-PA release significantly.
The effect of GBE on improving blood circulation may be partly attributed to its promoting TM expression and t-PA secretion by endothelial cells, and quercetin participated in the effect of GBE on t-PA secretion. However, the action of GBE on increasing TM expression needs further study.
为研究银杏叶提取物（GBE）改善血液循环的药理特性，研究了GBE及槲皮素（GBE中的主要黄酮类成分）对血栓调节蛋白（TM）表达和组织型纤溶酶原激活剂（t-PA）分泌的调节作用。
分别使用流式细胞仪和凝胶图像系统，体外评估人脐静脉内皮细胞（HUVECs）的TM表达和t-PA分泌。
GBE而非槲皮素以剂量和时间依赖性方式诱导HUVECs表面TM表达增加。GBE和槲皮素均显著增加t-PA释放。
GBE改善血液循环的作用可能部分归因于其促进内皮细胞TM表达和t-PA分泌，且槲皮素参与了GBE对t-PA分泌的作用。然而，GBE增加TM表达的作用尚需进一步研究。
