Singh Varsha, Belloir Christine, Siess Marie-Hélène, Le Bon Anne-Marie
UMR Toxicologie Alimentaire, Institut National de la Recherche Agronomique, France.
Nutr Cancer. 2006;55(2):178-84. doi: 10.1207/s15327914nc5502_9.
The present study was designed to investigate the protective efficiency of three garlic powders, obtained from bulbs grown in soils with different levels of sulfur fertilization, against DNA damage. Increasing fertilization of soil resulted in an increased alliin content of the powders. Garlic powders were administered to rats for 2 weeks (5% of the diet) and their antigenotoxic effects were examined in the liver and the colon using the comet assay. Consumption of the different garlic powders induced a 35-60% reduction in DNA damage induced by N-nitrosodimethylamine (NDMA) in rat liver. Increased alliin content of the garlic powder was associated strongly with a proportional decrease in NDMA induced DNA alteration. DNA damage induced by aflatoxin B1 in the liver or by 1,2-dimethylhydrazine in the colon were also decreased strongly by the three garlic powders but these decreases were not correlated to the alliin content of the garlic powders. Feeding garlic powders did not modify the genotoxic activity of the direct-acting carcinogen methylnitrosourea in the colon. Part of our results supports evidence that fertilization can have an impact on the protective capacity of garlic bulbs.
本研究旨在调查从生长于不同硫肥水平土壤中的鳞茎获得的三种蒜粉对DNA损伤的保护效果。土壤施肥量增加导致蒜粉中蒜氨酸含量增加。将蒜粉以5%的比例添加到大鼠饮食中，持续喂食2周，然后使用彗星试验检测其在肝脏和结肠中的抗遗传毒性作用。食用不同蒜粉可使大鼠肝脏中由N-亚硝基二甲胺（NDMA）诱导的DNA损伤减少35%至60%。蒜粉中蒜氨酸含量的增加与NDMA诱导的DNA改变成比例减少密切相关。三种蒜粉也能显著降低黄曲霉毒素B1在肝脏或1,2-二甲基肼在结肠中诱导的DNA损伤，但这些减少与蒜粉中蒜氨酸含量无关。喂食蒜粉不会改变直接作用致癌物甲基亚硝基脲在结肠中的遗传毒性活性。我们的部分研究结果支持了施肥会对大蒜鳞茎的保护能力产生影响这一证据。
