Ribeiro Marinei Cristina Pereira, Barbosa Nilda Berenice de Vargas, de Almeida Tielle Moraes, Parcianello Lutiane Mozzaquatro, Perottoni Juliano, de Avila Daiana Silva, Rocha João Batista Teixeira
Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Cell Biochem Funct. 2009 Oct;27(7):473-8. doi: 10.1002/cbf.1599.
This study evaluated the effect of possible synergic interaction between high fat diet (HF) and hydrochlorothiazide (HCTZ) on biochemical parameters of oxidative stress in brain. Rats were fed for 16 weeks with a control diet or with an HF, both supplemented with different doses of HCTZ (0.4, 1.0, and 4.0 g kg(-1) of diet). HF associated with HCTZ caused a significant increase in lipid peroxidation and blood glucose levels. In addition, HF ingestion was associated with an increase in cerebral lipid peroxidation, vitamin C and non-protein thiol groups (NPSH) levels. There was an increase in vitamin C as well as NPSH levels in HCTZ (1.0 and 4.0 g kg(-1) of diet) and HF plus HCTZ groups. Na(+)-K(+)-ATPase activity of HCTZ (4.0 g kg(-1) of diet) and HCTZ plus HF-fed animals was significantly inhibited. Our data indicate that chronic intake of a high dose of HCTZ (4 g kg(-1) of diet) or HF change biochemical indexes of oxidative stress in rat brain. Furthermore, high-fat diets consumption and HCTZ treatment have interactive effects on brain, showing that a long-term intake of high-fat diets can aggravate the toxicity of HCTZ.
本研究评估了高脂饮食（HF）与氢氯噻嗪（HCTZ）之间可能的协同相互作用对大脑氧化应激生化参数的影响。将大鼠分别用对照饮食或高脂饮食喂养16周，两种饮食均添加不同剂量的HCTZ（0.4、1.0和4.0 g kg⁻¹饮食）。HF与HCTZ联合使用导致脂质过氧化和血糖水平显著升高。此外，摄入HF与脑脂质过氧化、维生素C和非蛋白硫醇基团（NPSH）水平升高有关。在HCTZ（1.0和4.0 g kg⁻¹饮食）组以及HF加HCTZ组中，维生素C和NPSH水平均有所增加。HCTZ（4.0 g kg⁻¹饮食）组以及HCTZ加HF喂养的动物的Na⁺-K⁺-ATP酶活性受到显著抑制。我们的数据表明，长期摄入高剂量的HCTZ（4 g kg⁻¹饮食）或HF会改变大鼠大脑氧化应激的生化指标。此外，食用高脂饮食和HCTZ治疗对大脑有交互作用，表明长期摄入高脂饮食会加重HCTZ的毒性。
