Sachsenheimer W, Goody R S, Schirmer R H
Klin Wochenschr. 1975 Jul 1;53(13):617-22. doi: 10.1007/BF01469681.
Adenylate kinases, small organ-specific isoenzymes which appear after tissue damage in the blood plasma are partly eliminated via the kidney. After intravenous administration of 3000 enzyme units of 14C-labelled adenylate kinase to rats, about 50% of the enzyme and of the radioactivity are found in the urine within 7 minutes. The elimination of adenylate kinase from the serum occurs in two phases, a faster (half-life 16 minutes) and a slower (half-life 160 minutes). After intravenous adminstration of adenylate kinase to humans, a part of the activity was recovered in the urine within minutes. The potential use of assaying adenylate kinase levels for early diagnosis of myocardial infarction is discussed. Using various skeletal muscle diseases as examples, the possible use of the very rapid elimination of adenylate kinase from the serum in monitoring the course of the acute illnesses is described. The competitive inhibitor diadenosine pentaphosphate (AP5A) has a much higher affinity for the adenylate kinases from erythrocytes, heart or skeletal muscle than for the isoenzymes from liver or kidney. Therefore, AP5A can be used for the differential determination of adenylate kinase isoenzymes in the blood plasma or the urine.
腺苷酸激酶是一类小的器官特异性同工酶，在组织损伤后出现在血浆中，部分通过肾脏排出。给大鼠静脉注射3000酶单位的14C标记的腺苷酸激酶后，7分钟内约50%的酶和放射性物质出现在尿液中。血清中腺苷酸激酶的消除分两个阶段进行，一个较快阶段（半衰期16分钟）和一个较慢阶段（半衰期160分钟）。给人类静脉注射腺苷酸激酶后，几分钟内尿液中就可检测到部分活性。文中讨论了测定腺苷酸激酶水平用于早期诊断心肌梗死的潜在用途。以各种骨骼肌疾病为例，描述了利用血清中腺苷酸激酶快速消除的特性来监测急性疾病进程的可能用途。竞争性抑制剂二腺苷五磷酸（AP5A）对红细胞、心脏或骨骼肌中的腺苷酸激酶的亲和力比对肝脏或肾脏中的同工酶高得多。因此，AP5A可用于血浆或尿液中腺苷酸激酶同工酶的鉴别测定。
