从透析服务中的生物材料分离出的多重耐药凝固酶阴性葡萄球菌的DNA指纹图谱分析

DNA fingerprinting of a multi-resistant coagulase-negative staphylococci isolated from biomaterials in dialysis services.

作者信息

Chaieb Kamel, Touati Arabella, Salah Abbassi Mohamed, Hassen Assia Ben, Mahdouani Kacem, Bakhrouf Amina

机构信息

Laboratoire de Bactériologie de l'Hôpital Ibn El Jazzar de Kairouan, Tunisie.

出版信息

Arch Med Res. 2006 Nov;37(8):953-60. doi: 10.1016/j.arcmed.2006.06.007.

DOI:10.1016/j.arcmed.2006.06.007

PMID:17045110

Abstract

BACKGROUND

Coagulase-negative staphylococci (CoNS) have emerged as an important opportunistic pathogen isolated in hospital. Several species of CoNS have been implicated in human infections and disease especially in patients with poor health status.

METHODS

A total of 71 clinical strains of CoNS were isolated from dialysis fluid and needles in a dialysis unit and characterized. Susceptibility to antibiotics, biofilm production and molecular typing by pulsed-field gel electrophoresis (PFGE) were achieved.

RESULTS

The main isolated CoNS strains were Staphylococcus epidermidis (45%), Staphylococcus hominis (14%) and Staphylococcus haemolyticus (12.7%). The susceptibility profile of all strains revealed high resistance level to penicillin and oxacillin. PCR detection of oxacillin resistance gene (mecA gene) revealed a higher percentage of positive strains than the classic test (ATB Staph). Slime production test was positive in 60.6% of CoNS strains. PFGE analysis showed the presence of 69 restriction profiles clustered in 56 patterns.

CONCLUSIONS

Profiles of all isolates were generally heterogeneous, suggesting independent circulation with some evidence of cross-transmission.

摘要

背景

凝固酶阴性葡萄球菌（CoNS）已成为医院中分离出的一种重要的机会致病菌。几种CoNS菌株与人类感染和疾病有关，尤其是在健康状况较差的患者中。

方法

从一个透析单元的透析液和针头中分离出71株CoNS临床菌株并进行鉴定。测定了它们对抗生素的敏感性、生物膜形成情况以及通过脉冲场凝胶电泳（PFGE）进行分子分型。

结果

分离出的主要CoNS菌株为表皮葡萄球菌（45%）、人葡萄球菌（14%）和溶血葡萄球菌（12.7%）。所有菌株的药敏谱显示对青霉素和苯唑西林的耐药水平较高。对苯唑西林耐药基因（mecA基因）的PCR检测显示阳性菌株的比例高于传统检测（ATB Staph）。60.6%的CoNS菌株的黏液产生试验呈阳性。PFGE分析显示存在69种限制性图谱，聚为56种模式。