初治HIV感染患者中蛋白酶和逆转录酶耐药突变的频率
Frequency of protease and reverse transcriptase drug resistance mutations in naïve HIV-infected patients.
作者信息
Valle-Bahena Olivia M, Ramos-Jiménez Javier, Ortiz-López Rocío, Revol Agnès, Lugo-Trampe Angel, Barrera-Saldaña Hugo A, Rojas-Martínez Augusto
机构信息
Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, México.
出版信息
Arch Med Res. 2006 Nov;37(8):1022-7. doi: 10.1016/j.arcmed.2006.05.014.
BACKGROUND
Infections with drug-resistant HIV viruses in naïve subjects may cause antiretroviral (ARV) treatment failure. The prevalence of ARV resistance mutations in HIV-1 transcripts of infected naïve patients from northeast Mexico was determined in this study.
METHODS
RNA was extracted from plasma samples of 42 naïve individuals who were diagnosed between February 2001 and September 2003 as HIV-1 infected. Both protease (Pr) and reverse transcriptase (RT) were sequenced in 30 patients. In six samples only the RT segment was sequenced and in three samples only the protease segment was analyzed.
RESULTS
One of 36 isolates (2.8%) had the M184V resistance mutation to nucleoside retrotranscriptase inhibitors. In the Pr segment, only minor mutations were detected in 27/33 isolates (81.8%).
CONCLUSIONS
In this first study, prevalence of major mutations associated with ARV resistance in naïve patients in northeast Mexico is low compared to other countries, perhaps due to a low level of exposure of this population to ARV drugs.
背景
初治患者感染耐药性HIV病毒可能导致抗逆转录病毒（ARV）治疗失败。本研究确定了墨西哥东北部初治HIV感染患者的HIV-1转录本中ARV耐药突变的流行情况。
方法
从2001年2月至2003年9月期间诊断为HIV-1感染的42例初治个体的血浆样本中提取RNA。对30例患者的蛋白酶（Pr）和逆转录酶（RT）进行测序。在6个样本中仅对RT片段进行测序，在3个样本中仅分析蛋白酶片段。
结果
36株分离株中有1株（2.8%）对核苷类逆转录酶抑制剂具有M184V耐药突变。在Pr片段中，27/33株分离株（81.8%）仅检测到少量突变。
结论
在这项首次研究中，与其他国家相比，墨西哥东北部初治患者中与ARV耐药相关的主要突变流行率较低，这可能是由于该人群接触ARV药物的水平较低。
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