Song Byoung-Geun, Kim Tae-Kwon, Jung Young-Mi, Lee Yong-Hyun
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, S Korea.
J Biosci Bioeng. 2006 Sep;102(3):237-40. doi: 10.1263/jbb.102.237.
The talA gene encoding transaldolase, the key enzyme in the nonoxidative pentose phosphate pathway, was amplified in a transformant Escherichia coli harboring the phbCAB operon to shift the metabolic flux of the hexose mono-phosphate shunt to the odd-ball biosynthesis pathway for poly-beta-hydroxybutyrate overproduction. The PHB content in the transformant E. coli coharboring the phbCAB operon and talA gene increased from 28.2% to 52.3%, and the retarded cell growth was overcome. This increase seems to be mainly due to the concomitant supplies of the intermediates NADPH and acetyl-CoA, which are from the activated pentose phosphate pathway through the modulation of the talA gene and from the Embden-Meyerhof pathway.
编码转醛醇酶（非氧化磷酸戊糖途径中的关键酶）的talA基因，在携带phbCAB操纵子的转化型大肠杆菌中进行扩增，以将己糖单磷酸分流的代谢通量转移至用于过量生产聚-β-羟基丁酸酯的奇数碳生物合成途径。共携带phbCAB操纵子和talA基因的转化型大肠杆菌中的聚-β-羟基丁酸酯（PHB）含量从28.2%增加到52.3%，并且克服了细胞生长迟缓的问题。这种增加似乎主要归因于中间产物烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和乙酰辅酶A的同时供应，它们分别来自通过talA基因调控的活化磷酸戊糖途径以及糖酵解途径。
