谷氨酸棒杆菌生产可生物降解聚酯聚羟基丁酸酯的系统
Production system for biodegradable polyester polyhydroxybutyrate by Corynebacterium glutamicum.
作者信息
Jo Sung-Jin, Maeda Michihisa, Ooi Toshihiko, Taguchi Seiichi
机构信息
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Sapporo-shi, Hokkaido, Japan.
出版信息
J Biosci Bioeng. 2006 Sep;102(3):233-6. doi: 10.1263/jbb.102.233.
A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha. P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was microscopically observed as inclusion granules and its content was calculated to be 22.5% (w/w) with a number average molecular weight of 2.1x10(5) and a polydispersity of 1.63.
通过引入源自真养产碱菌的phbCAB操纵子，开发了一种谷氨酸棒杆菌生产聚（3-羟基丁酸酯）[P（3HB）]的生物合成途径。在携带细胞表面蛋白基因启动子的这种重组谷氨酸棒杆菌中检测到了P（3HB）合酶活性。通过显微镜观察到细胞内的P（3HB）为包涵体颗粒，其含量经计算为22.5%（w/w），数均分子量为2.1×10⁵，多分散性为1.63。
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