Fliegel L, Sardet C, Pouyssegur J, Barr A
Department of Pediatrics and Biochemistry, University of Alberta, Edmonton, Canada.
FEBS Lett. 1991 Feb 11;279(1):25-9. doi: 10.1016/0014-5793(91)80241-t.
We examined the myocardial form of the Na+/H+ exchanger. A partial length cDNA clone was isolated from a rabbit cardiac library and it encoded for a Na+/H+ exchange protein. In comparison with the human Na+/H+ exchanger, the sequence of the 5' end of the cDNA was highly conserved, much more than the 3' region, while the deduced amino acid sequence was also highly conserved. To further characterize the myocardial Na+/H+ exchange protein, we examined Western blots of isolated sarcolemma with antibody produced against a fusion protein of the Na+/H+ exchanger. The antibodies reacted with a sarcolemma protein of 50 kDa and with a protein of 70 kDa. The results show that the rabbit myocardium does possess a Na+/H+ exchanger protein homologous to the known human Na+/H+ exchanger.
我们研究了钠氢交换体的心肌形式。从兔心脏文库中分离出一个部分长度的cDNA克隆，它编码一种钠氢交换蛋白。与人类钠氢交换体相比，该cDNA 5'端的序列高度保守，远比3'区域保守，而推导的氨基酸序列也高度保守。为了进一步表征心肌钠氢交换蛋白，我们用针对钠氢交换体融合蛋白产生的抗体检测了分离的肌膜的免疫印迹。这些抗体与一个50 kDa的肌膜蛋白和一个70 kDa的蛋白发生反应。结果表明，兔心肌确实拥有一种与已知人类钠氢交换体同源的钠氢交换蛋白。
