Zhao Ying-yong, Cui Xiu-ming, Dai Yun, Miao Hua
Wenshan Prefecture Sanqi Science and Technology Research Institute, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(13):1056-8.
To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.
The chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (60:40). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).
The RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.
The method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.
