[Studies on HPLC fingerprint of Radix Aconiti Kusnezoffii].

OBJECTIVE

To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.

METHOD

The chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (60:40). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).

RESULT

The RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.

CONCLUSION

The method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.