[栀子对大鼠肝毒性的实验研究]
[Experimental studies on hepatotoxicity of rats induced by Fructus Gardeniae].
作者信息
Yang Hong-jun, Fu Mei-hong, Wu Zi-lun, Liang Ri-xing, Huang Lu-qi, Fang Jing, Li Geng, Cao Ying
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(13):1091-3.
OBJECTIVE
To study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae.
METHOD
Observe the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index. Detect the ALT, AST and TBIL. Observe the liver tissue by optical microscope.
RESULT
The weight and index of liver were increased by 3.08 g x kg(-1) aqueous extract, 1.62 g x kg(-1) alcoholic extract and 0.28 g x kg(-1) geniposide, compared to those of the blank group (P < 0.005, P < 0.001) and the activities of ALT, AST and the content of TBIL were also increased, compared to those of the blank group (P < 0.05, P < 0.001). The liver cells were obviously swell, necrotic and changed with inflammatory infiltrate.
CONCLUSION
Aqueous extract, alcoholic extract and geniposide displayed hepatotoxicity, and the geniposide which was the main substance of the Fructus Gardeniae might be mainly responsible for the hepatotoxicity.
目的
研究栀子不同提取物对大鼠的肝毒性作用。
方法
连续3天每日经口灌胃，观察大鼠外观、行为及体重变化。称量肝脏重量并计算肝脏指数。检测谷丙转氨酶（ALT）、谷草转氨酶（AST）和总胆红素（TBIL）。用光镜观察肝组织。
结果
与空白组相比，3.08 g·kg⁻¹水提取物、1.62 g·kg⁻¹醇提取物和0.28 g·kg⁻¹栀子苷可使肝脏重量和指数增加（P < 0.005，P < 0.001），ALT、AST活性及TBIL含量也升高（P < 0.05，P < 0.001）。肝细胞明显肿胀、坏死，并伴有炎性浸润。
结论
水提取物、醇提取物和栀子苷均表现出肝毒性，栀子苷作为栀子的主要成分可能是导致肝毒性的主要原因。
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