Liu Jian-xun, Han Xiao
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(10):829-31.
To study the time-effect, dose-effect of serum containing Shuangshentongguan (SSTG), a formula composed by active fraction of Chinese medicine.
Serum containing SSTG was obtained at different time after perorally administrated with different dose of SSTG. The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury and were treated by serum containing SSTG when reoxygenation. LDH content in supernatant was detected after reoxygenation. LDH release suppression rate was used to study the time-effect and dose-effect of serum containing SSTG.
The suppression rate of LDH release was not satisfied in serum containing high dose of SSTG. There was no significant dose-effect relationship between them. The suppression rate of LDH release was also different in drug serum taken at different time after SSTG treatment.
It was not the case that the higher the dose given to animal, the better the pharmacological effects.
研究含中药活性成分的双参通冠方（SSTG）血清的时效、量效关系。
给予不同剂量SSTG灌胃后，在不同时间点获取含SSTG血清。使心肌细胞缺氧缺糖以模拟缺氧复氧损伤，复氧时用含SSTG血清处理。复氧后检测上清液中乳酸脱氢酶（LDH）含量。用LDH释放抑制率研究含SSTG血清的时效和量效关系。
高剂量SSTG血清的LDH释放抑制率不理想。二者之间无显著量效关系。SSTG处理后不同时间采集的药物血清中LDH释放抑制率也不同。
并非给动物的剂量越高，药理作用就越好。
