Liu Jian-xun, Han Xiao, Xu Yong-gang, Ma Rou
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(12):995-8.
To explore the effects and mechanism of Shuangshen Tongguan (SSTG) serum, a formula composed of the active fractions of Chinese medicine, on calcium overload in cultured cardiomyocytes injured by hypoxia and reoxygenation.
The cardiomyocytes were deprived of oxygen and glucose to producl hypoxia reoxygenation injuried models. The changes of intracellular calcium fluorescence intensity induced by K+ and Thapsigargin were measured by fluorospectrophotometry and laster scanning confocal microscope respectively.
Intracellular calcium concentration was low in normal cardiomyocytes and was enhanced after hypoxia/reoxygenation (P < 0.05); SSTC drug serum reduced the intracellular calcium concentration and depressed the increase of calcium fluorescence intensity in singular cardiomyocyte due to K+ and Thapsigargin stimulation.
Hypoxia/reoxygenation injury, K+ and Thapsigargin could induce calcium overload in cardiomyocytes. The effects of calcium antagonism of SSTG drug serum were achieved by inhibiting calcium inflow, promoting calcium re-absorption of calcium.
探讨中药有效部位组方双参通冠（SSTG）血清对缺氧复氧损伤培养心肌细胞钙超载的影响及机制。
将心肌细胞进行缺氧缺糖处理以制备缺氧复氧损伤模型。分别采用荧光分光光度法和激光扫描共聚焦显微镜检测钾离子（K+）和毒胡萝卜素诱导的细胞内钙荧光强度变化。
正常心肌细胞内钙浓度较低，缺氧/复氧后升高（P < 0.05）；SSTC含药血清降低了细胞内钙浓度，并抑制了单个心肌细胞因K+和毒胡萝卜素刺激引起的钙荧光强度增加。
缺氧/复氧损伤、K+和毒胡萝卜素可诱导心肌细胞钙超载。SSTG含药血清的钙拮抗作用是通过抑制钙内流、促进钙重吸收实现的。
