Zhang Na, Xie Ming
Department on Formulaology, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(10):836-9.
To observe the renal injury in rats induced by Longdan Xiegan decoction (LDXGD) containing different dosages Aristolochia manshriensis.
SD rats were divided into four groups at random, and were fed with three kinds of LDXGD 13, 14.5, 17.5 g x kg(-1) (containing respectively A. manshriensis 1.5, 3, 6 g x kg(-1)) and distilled water respectively for 12 weeks. Renal functional parameters on 4,8,12 w were determined and changes of histomorphology in rats on the end of experiment were observed.
The LDXGD containing low dose (1.5 g x kg(-1)) A. manshriensis did't induce significantly renal injury in rats during 12 weeks; the LDXGD containing midst dose(3 g x kg(-1)) A. manshriensis induced light damage of proximal convoluted tubule epithelial cells in rats during 12 weeks; the LDXGD containing high dose(6 g x kg(-1)) A. manshriensis induced significantly renal injury in rats after administed 4 weeks. Along with the lasting of administration, the degree of injury became more seriously. The main renal injury location was in proximal convoluted tubule.
The renal toxicity of LDXGD is correlated with the dose of A. manshriensis and the time of administration. The LDXGD containing low dose A. manshriensis has relative security. However, the LDXGD containing high dose A. manshriensis can induce renal injury.
观察含不同剂量马兜铃的龙胆泻肝汤诱导大鼠肾损伤情况。
将SD大鼠随机分为四组，分别给予三种剂量13、14.5、17.5 g·kg⁻¹ 龙胆泻肝汤（分别含马兜铃1.5、3、6 g·kg⁻¹）及蒸馏水，连续灌胃12周。于4、8、12周测定大鼠肾功能指标，并观察实验结束时大鼠组织形态学变化。
含低剂量（1.5 g·kg⁻¹）马兜铃的龙胆泻肝汤在12周内未引起大鼠明显肾损伤；含中剂量（3 g·kg⁻¹）马兜铃的龙胆泻肝汤在12周内引起大鼠近端肾小管上皮细胞轻度损伤；含高剂量（6 g·kg⁻¹）马兜铃的龙胆泻肝汤在给药4周后引起大鼠明显肾损伤，且随着给药时间延长，损伤程度加重，主要肾损伤部位在近端肾小管。
龙胆泻肝汤的肾毒性与马兜铃剂量及给药时间相关，含低剂量马兜铃的龙胆泻肝汤相对安全，而含高剂量马兜铃的龙胆泻肝汤可致肾损伤。
