Wang Jin-Hua, Wang Zhi-Min, Jiang Xu, Xue Bao-Yun, Li Chun-Ying
Institute of Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700 China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):428-33.
To study the accumulated toxic action to bandicoot of aqueous extract of crude and processed Radix Aristolochice and the pharmacodynamic action of aqueous and alcoholic extract of crude and processed Radix Aristolochice.
The LD50 of acute toxicity to mice and chronic accumulated toxicity to bandicoots of crude and processed Radix Aristolochice were observated. Intestinal and myokinetic influence of normal and revulsive hyperactive gastrointestinal motility of mice induced by neostigmine were observated by giving aqueous extract and alcoholic extract of crude and processed Radix Aristolochice. Relieving pain and eliminating inflammation to mice also were observated.
The LD50 of aqueous extract of crude and processed Radix Aristolochice were 146. 45, 846.06 g X kg(-1) (equivalently to crude drug) respectively by intragastric administration. Bandicoot' general condition, peripheral blood, serum, organic coefficient, histopathologic examination weren't obvious changes after 1 month administrating aqueous extract of crude and processed drug in three dose. Serum indicators-urea nitrogen, cholesterol total, alkaline phosphatase manifestly were heightened and some animals'hepatic cells, nephric tubules and mucosa emerged differently damage at histomorphology by giving crude high dose after 2 months. Above organs emerged different damage in crude middle and high dose and processed high dose after 3 months and serum indicators- creatinine, urea nitrogen manifestly were increased, the coefficients of liver, kidney and gaster manifestly were heightened. However, the toxicity of identical dose processed product was lower than that of crude one. Aqueous extract and alcoholic extract of crude and processed Radix Aristolochice could obviously inhibite normal and revulsive hyperactive gastrointestinal motility by neostigmine of mice, relieve pain in mouse, stretching and heat stimulation models and inhibite dimethyl benzene-induc mouse, auricle inflammation. Pharmacodynamic action wasnt obvious difference in same dose of crude product and processed one.
Acute toxicity and chronic accumulated toxicity are stepped down after giving processed Radix Aristolochice, but pharmacodynamic effect wasn t lower. In pharmacodynamic effect, aqueous extract can't compare with alcoholic extract in same dose.
研究关木通生品与炮制品水提取物对大鼠的蓄积毒性作用以及关木通生品与炮制品水提取物和醇提取物的药效学作用。
观察关木通生品与炮制品对小鼠的急性毒性LD50和对大鼠的慢性蓄积毒性。给予关木通生品与炮制品水提取物和醇提取物，观察对正常小鼠及新斯的明引起的小鼠胃肠运动亢进的肠肌运动影响。观察对小鼠的镇痛、抗炎作用。
关木通生品与炮制品水提取物灌胃给药的LD50分别为146.45、846.06g·kg-1（相当于生药）。大鼠连续3个剂量给予关木通生品与炮制品水提取物1个月后，大鼠的一般状况、外周血、血清、脏器系数、组织病理学检查无明显变化。连续2个月给予关木通生品高剂量后，血清指标尿素氮、总胆固醇、碱性磷酸酶明显升高，部分动物肝细胞、肾小管及黏膜在组织形态学上出现不同程度损伤。连续3个月给予关木通生品中、高剂量及炮制品高剂量后，上述脏器出现不同程度损伤，血清指标肌酐、尿素氮明显升高，肝、肾、胃系数明显升高。但相同剂量炮制品的毒性低于生品。关木通生品与炮制品水提取物和醇提取物均能明显抑制正常小鼠及新斯的明引起的小鼠胃肠运动亢进，对小鼠扭体、热刺激模型有镇痛作用，对二甲苯致小鼠耳廓炎症有抑制作用。相同剂量生品与炮制品的药效学作用无明显差异。
关木通炮制品的急性毒性和慢性蓄积毒性降低，但药效学作用不降低。在药效学作用方面，相同剂量的水提取物不如醇提取物。
