Park Jun Hong, Oh Sun-Mee, Lim Soon Sung, Lee Yeon Sil, Shin Hyun-Kyung, Oh Yang-Seok, Choe Nong-Hoon, Park Jung Han Yoon, Kim Jin-Kyung
Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Gangwon-do 200-702, Republic of Korea.
Biochem Biophys Res Commun. 2006 Dec 8;351(1):146-52. doi: 10.1016/j.bbrc.2006.10.008. Epub 2006 Oct 10.
Foods of plant origin, especially fruits and vegetables, draw increased attention because of their potential benefits to human health. The aim of the present study was to determine in vitro anti-inflammatory activity of four different extracts obtained from the fruits of Rubus coreanus (aqueous and ethanol extracts of unripe and ripe fruits). Among the four extracts, the ethanol extract of unripe fruits of R. coreanus (URCE) suppressed nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. We also demonstrated that URCE by itself is a potent inducer of heme oxygenase-1 (HO-1). Inhibition of HO-1 activity by tin protoporphyrin, a specific HO-1 inhibitor, suppressed the URCE-induced reductions in the production of NO and PGE(2) as well as the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Our data suggest that URCE exerts anti-inflammatory effects in macrophages via activation of the HO-1 pathway and helps to elucidate the mechanism underlying the potential therapeutic value of R. coreanus extracts.
植物性食物，尤其是水果和蔬菜，因其对人类健康的潜在益处而受到越来越多的关注。本研究的目的是测定从高丽悬钩子果实中获得的四种不同提取物（未成熟和成熟果实的水提取物和乙醇提取物）的体外抗炎活性。在这四种提取物中，高丽悬钩子未成熟果实的乙醇提取物（URCE）抑制了脂多糖（LPS）刺激的RAW264.7小鼠巨噬细胞中一氧化氮（NO）和前列腺素E2（PGE2）的产生。我们还证明，URCE本身是血红素加氧酶-1（HO-1）的有效诱导剂。特异性HO-1抑制剂锡原卟啉对HO-1活性的抑制作用，抑制了URCE诱导的NO和PGE2产生的减少以及诱导型一氧化氮合酶（iNOS）和环氧化酶2（COX-2）的表达。我们的数据表明，URCE通过激活HO-1途径在巨噬细胞中发挥抗炎作用，并有助于阐明高丽悬钩子提取物潜在治疗价值的作用机制。
