Huang Shan, Meng Ning, Chang Bingquan, Quan Xianghua, Yuan RuiYing, Li Bin
1 Department of Pharmacy, Qingdao University of Science & Technology , Qingdao, China .
2 Department of Medicament, The Affiliated Hospital of Qingdao University , Qingdao, China .
J Med Food. 2018 Jul;21(7):726-733. doi: 10.1089/jmf.2017.4088. Epub 2018 Apr 5.
Epimedium brevicornu Maxim has been used as a traditional herbal drug in China. In this study, the anti-inflammatory effects of E. brevicornu Maxim ethanol extract (EBME) were investigated in RAW264.7 macrophages and mice challenged with lipopolysaccharide (LPS). Results showed that EBME attenuated inflammation by decreasing the production of several proinflammatory mediators, such as nitric oxide (NO), prostaglandin (PG) E, inducible nitric oxide synthase, and cyclooxygenase-2, in LPS-stimulated RAW264.7 macrophages. EBME increased the expression of heme oxygenase-1 (HO-1) and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2. The inhibitory effects of EBME on LPS-stimulated NO and PGE expression were partially reversed by HO-1 inhibitor. EBME also elicited an anti-inflammatory effect by inhibiting the production of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in LPS-induced peritonitis. Therefore, EBME exhibited anti-inflammatory effects in vitro and in vivo.
淫羊藿在中国一直被用作传统草药。在本研究中，研究了淫羊藿乙醇提取物（EBME）对RAW264.7巨噬细胞和脂多糖（LPS）攻击的小鼠的抗炎作用。结果表明，EBME通过减少几种促炎介质的产生来减轻炎症，这些促炎介质如一氧化氮（NO）、前列腺素（PG）E、诱导型一氧化氮合酶和环氧化酶-2，在LPS刺激的RAW264.7巨噬细胞中。EBME增加了血红素加氧酶-1（HO-1）的表达并促进了核因子红细胞2相关因子2的核转位。HO-1抑制剂部分逆转了EBME对LPS刺激的NO和PGE表达的抑制作用。EBME还通过抑制LPS诱导的腹膜炎中肿瘤坏死因子-α、白细胞介素（IL）-1β和IL-6的产生而发挥抗炎作用。因此，EBME在体外和体内均表现出抗炎作用。
