Arul Vadivel, Kartha Reena, Jayakumar Rajadas
Bio-Organic and Neurochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India.
Life Sci. 2007 Jan 2;80(4):275-84. doi: 10.1016/j.lfs.2006.09.018. Epub 2006 Sep 23.
Chronically elevated blood glucose levels result in reduced leukocyte function and cell malnutrition, which contribute to a high rate of wound infection and associated healing problems in diabetic patients. In the present study, the role of biotinylated GHK peptide (BioGHK) incorporated collagen biomaterial was tested for wound healing in diabetic rats. The rate of wound contraction and the levels of collagen, uronic acid, protein and DNA in the granulation tissue were determined. Further, the concentration of nitric oxide and other skin antioxidants was also monitored during the study. In diabetic rats treated with BioGHK incorporated collagen (Peptide Incorporated Collagen--PIC), the healing process was hastened with an increased rate of wound contraction. Glutathione (GSH) and ascorbic acid levels in the skin of streptozotocin-induced diabetic rats were higher in the PIC group as compared to control (Untreated) and collagen (Collagen Film--CF) treated groups. Superoxide dismutase (SOD) and catalase (CAT) activity was altered in all the groups. In vitro fibroblast cell culture studies suggest that PIC promotes fibroblast growth. Histological evaluation by haematoxylin-eosin and Masson's trichrome method revealed epithelialization, increased synthesis of collagen and activation of fibroblasts and mast cells in the PIC group. This study provides a rationale for the topical application of BioGHK incorporated collagen as a feasible and productive approach to support diabetic wound healing.
长期升高的血糖水平会导致白细胞功能降低和细胞营养不良，这使得糖尿病患者伤口感染率高且伴有相关愈合问题。在本研究中，测试了掺入生物素化甘氨酰 - 组氨酰 - 赖氨酸三肽（BioGHK）的胶原蛋白生物材料对糖尿病大鼠伤口愈合的作用。测定了伤口收缩率以及肉芽组织中胶原蛋白、糖醛酸、蛋白质和DNA的水平。此外，在研究过程中还监测了一氧化氮和其他皮肤抗氧化剂的浓度。在用掺入BioGHK的胶原蛋白（肽掺入胶原蛋白 - PIC）治疗的糖尿病大鼠中，伤口愈合过程加快，伤口收缩率增加。与对照组（未治疗）和胶原蛋白（胶原膜 - CF）治疗组相比，链脲佐菌素诱导的糖尿病大鼠皮肤中的谷胱甘肽（GSH）和抗坏血酸水平在PIC组中更高。所有组中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性均发生改变。体外成纤维细胞培养研究表明，PIC可促进成纤维细胞生长。苏木精 - 伊红染色和Masson三色法的组织学评估显示，PIC组出现上皮化、胶原蛋白合成增加以及成纤维细胞和肥大细胞活化。本研究为局部应用掺入BioGHK的胶原蛋白作为支持糖尿病伤口愈合的可行且有效的方法提供了理论依据。
