Rajasekaran N S, Nithya M, Rose C, Chandra T S
Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Madras, Chennai, Tamil Nadu.
Biochim Biophys Acta. 2004 Aug 4;1689(3):190-201. doi: 10.1016/j.bbadis.2004.03.004.
In the present study, the role of finger millet feeding on skin antioxidant status, nerve growth factor (NGF) production and wound healing parameters in healing impaired early diabetic rats is reported. Hyperglycemic rats received food containing 50 g/100 g finger millet (FM). Non-diabetic controls and diabetic controls received balanced nutritive diet. Full-thickness excision skin wounds were made after 2 weeks prior feeding of finger millet diet. The rate of wound contraction, and the levels of collagen, hexosamine and uronic acid in the granulation tissue were determined. The skin antioxidant status and lipid peroxide concentration were also monitored during the study. In hyperglycemic rats fed with finger millet diet, the healing process was hastened with an increased rate of wound contraction. Skin levels of glutathione (GSH), ascorbic acid and alpha-tocopherol in alloxan-induced diabetic rat were lower as compared to non-diabetics. Altered activities of superoxide dismutase (SOD) and catalase (CAT) were also recorded in diabetics. Interestingly, thiobarbituric acid reactive substances (TBARS) were elevated in the wound tissues of all the groups, when compared to normal (unwounded) skin tissues. However, in diabetic rats the TBARS levels of both normal and wounded skin tissues were significantly elevated (P < 0.001) when compared with control (non-diabetic) and diabetics fed with FM. Impaired production of NGF, determined by ELISA, in diabetic rats was improved upon FM feeding and further confirmed by immunocytochemical observations reflects the increased expression of NGF in hyperglycemic rats supplemented with FM-enriched diet. Histological and electron microscopical evaluations revealed the epithelialization, increased synthesis of collagen, activation of fibroblasts and mast cells in FM-fed animals. Thus, increased levels of oxidative stress markers accompanied by decreased levels of antioxidants play a vital role in delaying wound healing in diabetic rats. However, FM feeding to the diabetic animals, for 4 weeks, controlled the glucose levels and improved the antioxidant status, which hastened the dermal wound healing process.
在本研究中，报告了食用黍米对早期糖尿病大鼠皮肤抗氧化状态、神经生长因子（NGF）产生及伤口愈合参数在愈合受损方面的作用。高血糖大鼠食用含50 g/100 g黍米（FM）的食物。非糖尿病对照组和糖尿病对照组接受均衡营养饮食。在喂食黍米饮食2周后进行全层切除皮肤伤口。测定伤口收缩率以及肉芽组织中胶原蛋白、氨基葡萄糖和糖醛酸的水平。在研究期间还监测了皮肤抗氧化状态和脂质过氧化物浓度。在喂食黍米饮食的高血糖大鼠中，伤口收缩率增加，愈合过程加快。与非糖尿病大鼠相比，四氧嘧啶诱导的糖尿病大鼠皮肤中谷胱甘肽（GSH）、抗坏血酸和α-生育酚水平较低。糖尿病大鼠中还记录到超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性改变。有趣的是，与正常（未受伤）皮肤组织相比，所有组的伤口组织中硫代巴比妥酸反应性物质（TBARS）均升高。然而，与对照组（非糖尿病）和喂食FM的糖尿病大鼠相比，糖尿病大鼠正常和受伤皮肤组织的TBARS水平均显著升高（P < 0.001）。通过ELISA测定，糖尿病大鼠中NGF产生受损在喂食FM后得到改善，免疫细胞化学观察进一步证实，这反映了补充富含FM饮食的高血糖大鼠中NGF表达增加。组织学和电子显微镜评估显示，喂食FM的动物出现上皮形成、胶原蛋白合成增加、成纤维细胞和肥大细胞活化。因此，氧化应激标志物水平升高伴随抗氧化剂水平降低在糖尿病大鼠伤口愈合延迟中起重要作用。然而，给糖尿病动物喂食FM 4周可控制血糖水平并改善抗氧化状态，从而加速皮肤伤口愈合过程。
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