Sun Yun, Collett Jon, Fullwood Nigel J, Mac Neil Sheila, Rimmer S
Chemistry Department, University of Sheffield, Sheffield, South Yorkshire S3 7HF, UK.
Biomaterials. 2007 Feb;28(4):661-70. doi: 10.1016/j.biomaterials.2006.09.024. Epub 2006 Oct 16.
Amphiphilic block terpolymer conetworks composed of butyl methacrylate (BMA), 2,3 propandiol-1-methacrylate (GMMA) and ethandiol dimethacrylate (EDMA) were synthesized. Telechelic oligomers with the carboxylic acid end groups were made via ozonolysis of poly(BMA-co-butadiene) and then these were reacted with glycidyl methacrylate to obtain cross-linkable vinyl groups at both chain ends. Networks were then formed via free radical copolymerization with EDMA and GMMA or 2-methyl-acrylic acid 2,2-dimethyl-[1,3]dioxolan-4-ylmethyl ester (GMAc). The acetonide groups of the GMAc units were then removed, by reaction with selenium dioxide and hydrogen peroxide, to give networks with the same molecular structure as the GMMA terpolymers but different cell adhesion and protein adsorption properties. Protein adsorption was maximised in networks prepared with GMMA rather than with GMAc followed by removal of the acetonide. Block conetworks that were synthesised with GMAc were poor substrates for cell proliferation whilst the GMMA class support good levels of both cell viability and proliferation. It is suggested that the difference in behaviour is derived from changes in the surface composition.
合成了由甲基丙烯酸丁酯（BMA）、甲基丙烯酸-2,3-丙二醇酯（GMMA）和乙二醇二甲基丙烯酸酯（EDMA）组成的两亲性嵌段三元共聚物网络。通过聚（BMA-共-丁二烯）的臭氧分解制备具有羧酸端基的遥爪低聚物，然后使其与甲基丙烯酸缩水甘油酯反应，在链两端获得可交联的乙烯基。然后通过与EDMA和GMMA或2-甲基丙烯酸-2,2-二甲基-[1,3]二氧戊环-4-基甲酯（GMAc）进行自由基共聚形成网络。然后通过与二氧化硒和过氧化氢反应去除GMAc单元的丙酮叉基团，得到具有与GMMA三元共聚物相同分子结构但具有不同细胞粘附和蛋白质吸附特性的网络。用GMMA制备的网络中蛋白质吸附最大化，而不是用GMAc制备然后去除丙酮叉基团的网络。用GMAc合成的嵌段网络是细胞增殖的不良底物，而GMMA类支持良好水平的细胞活力和增殖。有人认为行为上的差异源于表面组成的变化。
