Laboratory for Polymer Analysis, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India.
J Mater Sci Mater Med. 2009 Dec;20 Suppl 1:S49-60. doi: 10.1007/s10856-008-3481-7. Epub 2008 Jun 17.
A protein based 3D porous scaffold is fabricated by blending gelatin and albumin. The biomimetic biodegradable gelatin, promoted good cell adhesion and its hydrophilic nature enabled absorption of culture media. Albumin is proposed to serve as a nontoxic foaming agent and also helped to attain a hydrophobic-hydrophilic balance. The hydrophobic-hydrophilic balance and appropriate crosslinking of the scaffold avoided extensive swelling, as well as retained the stability of scaffold in culture medium for long period. The scaffold is found to be highly porous with open interconnected pores. The adequate swelling and mechanical property of the scaffold helped to withstand the loads imparted by the cells during in vitro culture. The scaffold served as a nontoxic material to monolayer of fibroblast cells and is found to be cell compatible. The suitability of scaffold for chondrocyte culture and stem cell differentiation to chondrocytes is further explored in this work. The scaffold provided appropriate environment for chondrocyte culture, resulting in deposition of cartilage specific matrix molecules that completely masked the pores of the porous scaffold. The scaffold promoted the proliferation and differentiation of mesenchymal stem cells to chondrocytes in presence of growth factors. The transforming growth factor, TGFbeta3 promoted better chondrogenic differentiation than its isoform TGFbeta1 in this scaffold.
通过将明胶和白蛋白混合，制备了一种基于蛋白质的 3D 多孔支架。仿生可生物降解的明胶促进了良好的细胞黏附，其亲水性使其能够吸收培养基。白蛋白被提议用作无毒的发泡剂，还有助于实现疏水-亲水平衡。疏水-亲水平衡和支架的适当交联避免了广泛的肿胀，并保持了支架在培养基中的长期稳定性。该支架具有高度多孔的结构，具有开放的互连孔。支架具有适当的溶胀性和机械性能，有助于承受体外培养过程中细胞施加的负载。支架被证明是一种对成纤维细胞单层无毒的材料，并且与细胞相容。本工作进一步探讨了支架用于软骨细胞培养和干细胞向软骨细胞分化的适宜性。支架为软骨细胞培养提供了合适的环境，导致软骨特异性基质分子的沉积，完全掩盖了多孔支架的孔。在生长因子存在的情况下，支架促进间充质干细胞向软骨细胞的增殖和分化。在该支架中，转化生长因子 TGFbeta3 比其同工型 TGFbeta1 更能促进更好的软骨分化。
