Denèfle J P, Zhu Q L
Centre de Biologie Cellulaire du C.N.R.S., Ivry-sur-Seine, France.
Tissue Cell. 1990;22(5):575-81. doi: 10.1016/0040-8166(90)90056-f.
Single and double-label immunofluorescence were used to study the fibronectin (FN) and keratins (Ks) localization patterns in early wounded confluent PtK2 cells. A time-course study (0 hr, 2 hr, 6 hr and 24 hr) gives the following results: before wounding, the FN localizations of confluent cells are composed of curved and sometimes branched strands or fibrils. The Ks network is formed by radial fluorescent filaments connecting the Ks centers near the nuclei with a linear fluorescence underlying the cell membrane. Two hr after, the FN localizations are redistributed at the cell-cell contact areas. The radial Ks filaments are compacted around the nuclei, some of them delineate the cytoplasmic periphery of the wounded cells. Six hr later, the method shows redistributed FN localizations at the cell-cell contact areas. An alveolar pattern is formed enclosing each of the adjacent cells. The codetected Ks filaments are retracted around the nuclei. The underlying cell-cell contact areas are also well demonstrated. It may be noted that these areas are FN-labelled. Twenty-four hr after wounding, the FN alveolar pattern persists. The redistributed Ks filaments have some similarity to those seen before wounding.
采用单标和双标免疫荧光法研究早期受伤的汇合PtK2细胞中纤连蛋白（FN）和角蛋白（Ks）的定位模式。一项时间进程研究（0小时、2小时、6小时和24小时）给出了以下结果：受伤前，汇合细胞的FN定位由弯曲且有时分支的链或纤维组成。Ks网络由放射状荧光丝形成，这些荧光丝将细胞核附近的Ks中心与细胞膜下方的线性荧光连接起来。两小时后，FN定位重新分布在细胞-细胞接触区域。放射状的Ks丝在细胞核周围聚集，其中一些描绘了受伤细胞的细胞质周边。六小时后，该方法显示FN定位在细胞-细胞接触区域重新分布。形成了包围每个相邻细胞的肺泡样模式。共检测到的Ks丝在细胞核周围回缩。细胞-细胞接触的下层区域也得到了很好的显示。值得注意的是，这些区域被FN标记。受伤24小时后，FN肺泡样模式持续存在。重新分布的Ks丝与受伤前观察到的有一些相似之处。
