Zhu Q L, Denèfle J P
Centre de Biologie Cellulaire CNRS, Ivry-sur-Seine, France.
Tissue Cell. 1989;21(5):653-60. doi: 10.1016/0040-8166(89)90076-1.
A confluent PtK2 cell sheet was incised in a serum-free culture medium, at 15 min, 2 hr and 24 hr after wounding. The culture media were collected in the same way and used as conditioned media. Unwounded confluent cells were cultured in the conditioned medium for 24 hr. They showed a modification of fibronectin localization similar to that which we had previously observed in wounded confluent PtK2 cells: cells lost their normal fibronectin fibrils and were surrounded by fibronectin lace. This finding suggested that during wound healing, the cells released soluble chemical factors which could modify the fibronectin localization pattern of unwounded confluent cells. Subconfluent cells did not respond to conditioned media, showing that confluent cells and subconfluent cells had different susceptibilities.
在无血清培养基中，于损伤后15分钟、2小时和24小时对汇合的PtK2细胞片进行切割。以相同方式收集培养基并用作条件培养基。将未损伤的汇合细胞在条件培养基中培养24小时。它们显示出纤连蛋白定位的改变，类似于我们之前在损伤的汇合PtK2细胞中观察到的情况：细胞失去了正常的纤连蛋白原纤维，被纤连蛋白网所包围。这一发现表明，在伤口愈合过程中，细胞释放出可溶性化学因子，这些因子可以改变未损伤汇合细胞的纤连蛋白定位模式。亚汇合细胞对条件培养基没有反应，表明汇合细胞和亚汇合细胞具有不同的敏感性。
