Denèfle J P, Zhu Q L
Centre de Biologie Cellulaire CNRS, Ivry-sur-Seine, France.
Tissue Cell. 1989;21(5):647-51. doi: 10.1016/0040-8166(89)90075-x.
Confluent PtK2 cells 4 hr treated with 5 mM acrylamide were FN-detected by indirect immunofluorescence. The initial fibrillar-FN network was replaced by an alveolar-type network located at the cell-cell contacts areas in the form of a thick frame with a lace-like appearance. Afterwards, acrylamide removal was obtained by several washes with fresh FCS-free culture medium. Then, PtK2 cells were returned to the incubator for 20 hr. Cell recovery was indicated by reversion of the initial fibrillar-FN network. These data show that FN reversion was possible without any changes in shape and cytoplasmic organization of non-motile growing cells.
用5 mM丙烯酰胺处理4小时的融合PtK2细胞通过间接免疫荧光检测纤连蛋白（FN）。最初的纤维状FN网络被一种肺泡型网络所取代，该网络以厚框架形式位于细胞-细胞接触区域，呈花边状外观。之后，通过用不含新鲜胎牛血清（FCS）的培养基多次洗涤去除丙烯酰胺。然后，将PtK2细胞放回培养箱中培养20小时。初始纤维状FN网络的恢复表明细胞恢复。这些数据表明，在非运动性生长细胞的形状和细胞质组织没有任何变化的情况下，FN恢复是可能的。
