Furuya S, Edwards C, Ornberg R
National Institute for Physiological Sciences, Okazaki, Japan.
Tissue Cell. 1990;22(5):615-28. doi: 10.1016/0040-8166(90)90059-i.
Bovine adrenal medulla capillary endothelial cells were isolated and cloned, and their morphological behaviors in vitro were examined. In the culture of primary or early passage, one type of colony formed intracellular lumina both on the dish and in the three dimensional collagen gel. Another type proliferated well and showed morphology ranging from slender-shape to cobblestone shape, and were easily cloned. Cloned cells which showed slender-shapes formed tubular network on plastic dish after addition of PMA, OAG or vanadate, and these cells also formed multicellular tubules in the three dimensional collagen gel. However, the formation of diaphragmed fenestrae by these slender-shape clones was rare. One clone which showed cobblestone shape formed diaphragmed fenestrae, when cultured on collagen gel for more than one month. Isolated colonies or clones showed heterogeneity of cell shape, angiogenic behaviors and fenestrae formation.
分离并克隆了牛肾上腺髓质毛细血管内皮细胞，并检测了它们在体外的形态学行为。在原代培养或早期传代培养中，一种集落类型在培养皿和三维胶原凝胶中均形成细胞内管腔。另一种类型增殖良好，形态从细长形到鹅卵石形不等，且易于克隆。呈细长形的克隆细胞在添加佛波酯（PMA）、1-油酰基-2-乙酰基-sn-甘油（OAG）或钒酸盐后在塑料培养皿上形成管状网络，并且这些细胞在三维胶原凝胶中也形成多细胞小管。然而，这些细长形克隆形成有隔膜的窗孔很少见。一个呈鹅卵石形的克隆在胶原凝胶上培养一个多月后形成了有隔膜的窗孔。分离的集落或克隆显示出细胞形状、血管生成行为和窗孔形成的异质性。
