Wakabayashi T, Onoda H
Central Clinic Laboratory, University of Tokyo, Japan.
Virchows Arch A Pathol Anat Histopathol. 1991;418(2):105-10. doi: 10.1007/BF01600285.
Seventeen human renal graft biopsies taken 1 h to 50 days after transplantation and 3 human renal non-graft biopsies (2 minimal change and 1 non-tumour portion of angiomyolipoma) were investigated with immunoelectron microscopy in order to identify interdigitating reticulum cells (IDC) or dendritic cells (DC) in renal tissues. The antibodies used consisted of a rabbit polyclonal antibody of antihuman S100 beta protein, mouse monoclonal antibodies of antihuman HLA-DR, anti-CD3, and anti-CD1a. IDC or DC were identified in 11 renal grafts. They were found both in the glomerular and interstitial (peritubular) capillary lumens but not in the interstitium of 1 case: both were present in the interstitial capillary lumens and interstitium of another case, and in the interstitium only of 9 cases. In the remaining 6 grafts and 3 non-grafts they were not detected. These 6 grafts and 3 non-grafts did not show any pathological change except for foot process fusion of the glomerular epithelia in 2 cases of minimal change. These findings suggest that IDC or DC are not normally present in human renal tissues. The presence of the cell in the glomerular and peritubular capillary lumens of a biopsy taken after 1 h and their presence in the interstitial capillary lumens of another graft biopsy, suggest that the IDC or DC in human renal grafts are derived from recipients, not donors, and that they migrate from the circulating blood toward the interstitium.
为了在肾组织中识别交错突网状细胞（IDC）或树突状细胞（DC），对17例移植后1小时至50天的人肾移植活检组织以及3例人肾非移植活检组织（2例微小病变和1例血管平滑肌脂肪瘤的非肿瘤部分）进行了免疫电子显微镜检查。所使用的抗体包括兔抗人S100β蛋白多克隆抗体、鼠抗人HLA-DR、抗CD3和抗CD1a单克隆抗体。在11例肾移植中识别出了IDC或DC。它们见于肾小球和间质（肾小管周围）毛细血管腔内，但有1例在间质中未发现：在另一例中两者均见于间质毛细血管腔和间质，而在9例中仅见于间质。在其余6例移植组织和3例非移植组织中未检测到它们。除2例微小病变的肾小球上皮足突融合外，这6例移植组织和3例非移植组织未显示任何病理变化。这些发现提示，IDC或DC在人肾组织中通常不存在。在移植后1小时获取的活检组织的肾小球和肾小管周围毛细血管腔内存在这种细胞，以及在另一例移植活检组织的间质毛细血管腔内存在这种细胞，提示人肾移植中的IDC或DC来源于受者而非供者，并且它们从循环血液向间质迁移。
