Lemire J M, Archer D C
Department of Pediatrics, University of Texas Medical School, Houston 77030.
J Clin Invest. 1991 Mar;87(3):1103-7. doi: 10.1172/JCI115072.
The hormone, 1,25-dihydroxyvitamin D3 (1,25-[OH]2-D3), inhibits lymphocyte activation in vitro. We studied the ability of the vitamin D metabolite to interfere in vivo with a primary T cell-mediated model of autoimmunity, murine experimental autoimmune encephalomyelitis (EAE). Within 2 wk of antigenic challenge, immunized animals will develop acute paralysis with central nervous tissue inflammation. If mice survive, a rise in antibody titer develops within a month. The administration of 0.1 microgram 1,25-(OH)2-D3 i.p. given every other day for 15 d, starting 3 d before immunization, significantly prevented the development of EAE. The rise in antibody titer to myelin basic protein was also abrogated. Histopathologic lesions of EAE were inhibited by treatment with the sterol. These results suggest a potent immunosuppressive role for 1,25-(OH)2-D3 in vivo in the modulation of a cell-mediated model of autoimmunity.
激素1,25 - 二羟基维生素D3（1,25 - [OH]2 - D3）在体外可抑制淋巴细胞活化。我们研究了这种维生素D代谢产物在体内干扰原发性T细胞介导的自身免疫模型——小鼠实验性自身免疫性脑脊髓炎（EAE）的能力。在抗原攻击后2周内，免疫动物会出现伴有中枢神经组织炎症的急性麻痹。如果小鼠存活下来，一个月内抗体滴度会升高。从免疫前3天开始，每隔一天腹腔注射0.1微克1,25 - (OH)2 - D3，持续15天，可显著预防EAE的发生。针对髓鞘碱性蛋白的抗体滴度升高也被消除。用该固醇类物质治疗可抑制EAE的组织病理学损伤。这些结果表明，1,25 - (OH)2 - D3在体内对细胞介导的自身免疫模型的调节中具有强大的免疫抑制作用。
