Elde R, Schalling M, Ceccatelli S, Nakanishi S, Hökfelt T
Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.
Neurosci Lett. 1990 Nov 27;120(1):134-8. doi: 10.1016/0304-3940(90)90187-e.
The expression of receptors for neurotensin and substance P was examined in rat brain and spinal cord using in situ hybridization with synthetic oligonucleotide probes. Strong hybridization signals for neurotensin receptor mRNA were observed over neurons i.a. in the diagonal band, medial septal nucleus, nucleus basalis magnocellularis, suprachiasmatic nucleus, supramammillary area, substantia nigra and ventral tegmental area. Strong hybridization signals for substance P receptor mRNA were observed over scattered, large neurons in the striatum, and in the spinal cord over neurons in the dorsal horn, the area around the central canal and preganglionic autonomic neurons. Thus, discrete neurons in several brain regions express a G-protein-coupled receptor with which endogenous neurotensin and substance P may interact.
利用合成寡核苷酸探针原位杂交技术，检测了大鼠脑和脊髓中神经降压素和P物质受体的表达。在多个脑区的神经元上观察到神经降压素受体mRNA的强杂交信号，如斜角带、内侧隔核、大细胞基底核、视交叉上核、乳头体上区、黑质和腹侧被盖区。在纹状体中散在的大神经元上以及脊髓背角、中央管周围区域和节前自主神经元上观察到P物质受体mRNA的强杂交信号。因此，几个脑区中的离散神经元表达一种G蛋白偶联受体，内源性神经降压素和P物质可能与其相互作用。
