Wu Trang-Tiau, Hsieh Yi-Hsien, Wu Cheng-Chung, Tsai Jen-Hsiang, Hsieh Yih-Shou, Huang Chih-Yang, Liu Jer-Yuh
Department of Surgery, School of Medicine, Medical College, Chung Shan Medical University Taichung 402, Taiwan, ROC.
Chin J Physiol. 2006 Aug 31;49(4):192-8.
To compare gene expression patterns between the poorly-differentiated (HA22T/VGH) and well-differentiated (HepG2) hepatocellular carcinoma cells, messenger RNA was isolated form both kinds of cells and subjected to differential displays reversing transcriptase-polymerase chain reaction (DDRT-PCR) technology. Gene fragments showing difference in the expression were recovered, reamplified, cloned and sequenced. Anion exchanger 2 (AE2), an isoform of band 3 protein, was identified and chosen for further characterization. AE2 was strongly expressed in HA22T/VGH cells, while it was little expressed in the well-differentiated hepatocellular carcinoma cells (PLC/PRF5 and HepG2) or little in the normal liver cell (Chang liver). In the 28 pairs of HCC and adjacent non-tumor tissues, levels in the cancer tissue (32.7 +/- 5.0) were significantly higher than that in the adjacent non-tumor tissue (9.1 +/- 2.4) (P < 0.01). Moreover, 19 cases (68%) showed over-expression of AE2 in HCC tissues, 3 cases were similar in both tissues, and 6 cases exhibited little or undetectable signals. Twenty cases (71%) of adjacent normal tissue showed little or undetectable signals. The results indicated that overexpression of AE2 may be involved in the development of human HCC.
为比较低分化（HA22T/VGH）和高分化（HepG2）肝癌细胞之间的基因表达模式，从这两种细胞中分离信使核糖核酸，并采用差异显示反转录-聚合酶链反应（DDRT-PCR）技术。回收、重新扩增、克隆并测序显示表达差异的基因片段。鉴定并选择带3蛋白的一种同工型阴离子交换蛋白2（AE2）进行进一步特性分析。AE2在HA22T/VGH细胞中强烈表达，而在高分化肝癌细胞（PLC/PRF5和HepG2）中表达很少，在正常肝细胞（Chang liver）中几乎不表达。在28对肝癌组织及相邻非肿瘤组织中，癌组织中的水平（32.7±5.0）显著高于相邻非肿瘤组织（9.1±2.4）（P<0.01）。此外，19例（68%）肝癌组织中AE2呈过表达，3例在两种组织中相似，6例信号很少或检测不到。20例（71%）相邻正常组织信号很少或检测不到。结果表明，AE2过表达可能参与人类肝癌的发生发展。
