Tan Bo, Ma Yueming, Shi Rong, Wang Tianming
Laboratory of Pharmacokinetics, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
Biopharm Drug Dispos. 2007 Dec;28(9):511-6. doi: 10.1002/bdd.578.
A high-performance liquid chromatographic method with ultraviolet detection was established and validated for quantification of three alkaloids (coptisine, palmatine and berberine) in rat urine. Following a single-step liquid-liquid extraction, the analytes were separated on a reversed-phase C(18) column with water-formic acid-triethylamine-methanol as the mobile phase at a flow rate of 1 ml/min. The linear ranges of the calibration curves were 1.6-160 ng/ml for all three alkaloids. The lower limit of quantification was 1.6 ng/ml for all three alkaloids. The within-batch accuracy was 90.4-108.3% for coptisine, 88.6-107.8% for berberine and 88.4-110.1% for palmatine. The between-batch accuracy was 99.3-100.3% for coptisine, 94.3-100.6% for berberine and 93.7-100.0% for palmatine. The within-batch and between-batch precisions were <or=10.1% and <or=11.1%, respectively. This method is suitable to determine the three alkaloids of Coptidis Rhizoma in urine simultaneously and thus to investigate the pharmacokinetics of the alkaloids from Xiexin Decoction in rats.
建立了一种带紫外检测的高效液相色谱法，并对其进行验证，用于定量大鼠尿液中的三种生物碱（黄连碱、巴马汀和小檗碱）。经过一步液液萃取后，分析物在反相C(18)柱上分离，流动相为水 - 甲酸 - 三乙胺 - 甲醇，流速为1 ml/min。所有三种生物碱校准曲线的线性范围均为1.6 - 160 ng/ml。所有三种生物碱的定量下限均为1.6 ng/ml。黄连碱的批内准确度为90.4 - 108.3%，小檗碱为88.6 - 107.8%，巴马汀为88.4 - 110.1%。黄连碱的批间准确度为99.3 - 100.3%，小檗碱为94.3 - 100.6%，巴马汀为93.7 - 100.0%。批内和批间精密度分别≤10.1%和≤11.1%。该方法适用于同时测定尿液中黄连的三种生物碱，从而研究泻心汤中生物碱在大鼠体内的药代动力学。
