补骨脂素紫外线A疗法可增加小鼠皮肤中鸟氨酸脱羧酶基因的表达。

PUVA increases ornithine decarboxylase gene expression in mouse skin.

作者信息

Kono T, Mizuno N, Taniguchi S, Ishii M, Yoshida H, Matsui-Yuasa I, Otani S, Hamada T

机构信息

Department of Dermatology, Osaka City University Medical School, Japan.

出版信息

Dermatologica. 1990;181(4):334-7. doi: 10.1159/000247842.

DOI:10.1159/000247842

PMID:1705904

Abstract

The influence of topical PUVA on the activity of ornithine decarboxylase (ODC) and its gene expression was investigated in the skin of hairless mouse. After 1 h of application of 0.3% 8-methoxypsoralen, irradiation of 3 J/cm2 of ultraviolet A (UV-A) was administered. The ODC activity markedly increased and peaked at 24 h following UV-A irradiation. The ODC mRNA level, analyzed in dot-blot analysis, elevated to about 5 times that of control at 24 h after treatment. These results show that the PUVA-induced ODC activity is due in part to an increase in ODC gene expression.

摘要

研究了局部补骨脂素紫外线A光化学疗法（PUVA）对无毛小鼠皮肤中鸟氨酸脱羧酶（ODC）活性及其基因表达的影响。在涂抹0.3%的8-甲氧基补骨脂素1小时后，给予3 J/cm2的紫外线A（UV-A）照射。ODC活性显著增加，并在UV-A照射后24小时达到峰值。斑点印迹分析显示，治疗后24小时，ODC mRNA水平升高至对照的约5倍。这些结果表明，PUVA诱导的ODC活性部分归因于ODC基因表达的增加。