Butter J Y P, Hecht B, Crenshaw B R, Weder C
Nano-Optics Group, National Center of Competence for Research in Nanoscale Science, Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland.
J Chem Phys. 2006 Oct 21;125(15):154710. doi: 10.1063/1.2360268.
Simultaneous detection of single molecules by absorption and fluorescence is demonstrated using confocal microscopy at cryogenic temperature. Dynamical processes such as blinking and spectral jumping of single emitters are observed in both detection channels. The relative magnitude of fluorescence and absorption varies between molecules. In particular, we observe molecules that do not emit detectable Stokes-shifted fluorescence but show a strong absorption signal. The fact that coherent resonant scattering underlies the absorption process is demonstrated by a correlation between small linewidth and large absorption amplitude.
利用低温共聚焦显微镜实现了通过吸收和荧光同时检测单分子。在两个检测通道中都观察到了单发射体的闪烁和光谱跳跃等动态过程。不同分子的荧光和吸收的相对强度有所不同。特别是，我们观察到一些分子不发射可检测到的斯托克斯位移荧光，但显示出很强的吸收信号。小线宽和大吸收幅度之间的相关性证明了吸收过程的基础是相干共振散射。
