Thiel Vera, Neulinger Sven C, Staufenberger Tim, Schmaljohann Rolf, Imhoff Johannes F
Leibniz-Institut für Meereswissenschaften, IFM-GEOMAR, Kiel, Germany.
FEMS Microbiol Ecol. 2007 Jan;59(1):47-63. doi: 10.1111/j.1574-6941.2006.00217.x. Epub 2006 Oct 24.
The local distribution of the bacterial community associated with the marine sponge Tethya aurantium Pallas 1766 was studied. Distinct bacterial communities were found to inhabit the endosome and cortex. Clear differences in the associated bacterial populations were demonstrated by denaturing gradient gel electrophoresis (DGGE) and analysis of 16S rRNA gene clone libraries. Specifically associated phylotypes were identified for both regions: a new phylotype of Flexibacteria was recovered only from the sponge cortex, while Synechococcus species were present mainly in the sponge endosome. Light conduction via radiate spicule bundles conceivably facilitates the unusual association of Cyanobacteria with the sponge endosome. Furthermore, a new monophyletic cluster of sponge-derived 16S rRNA gene sequences related to the Betaproteobacteria was identified using analysis of 16S rRNA gene clone libraries. Members of this cluster were specifically associated with both cortex and endosome of T. aurantium.
对与1766年帕拉斯的海洋海绵橙黄桶海绵相关的细菌群落的局部分布进行了研究。发现不同的细菌群落栖息在内质和皮层中。通过变性梯度凝胶电泳（DGGE）和16S rRNA基因克隆文库分析证明了相关细菌种群存在明显差异。为这两个区域鉴定了特定相关的系统型：仅从海绵皮层中分离出一种新的柔膜菌系统型，而聚球藻属物种主要存在于海绵内质中。通过辐射状骨针束传导光线可以想象促进了蓝细菌与海绵内质的异常关联。此外，使用16S rRNA基因克隆文库分析鉴定了一个与β-变形菌相关的新的海绵衍生16S rRNA基因序列单系簇。该簇的成员与橙黄桶海绵的皮层和内质均有特定关联。
