Bej A K, Mahbubani M H, Atlas R M
Department of Biology, University of Louisville, Kentucky 40292.
Appl Environ Microbiol. 1991 Feb;57(2):597-600. doi: 10.1128/aem.57.2.597-600.1991.
Methods using polymerase chain reaction (PCR) and gene probes to detect viable Legionella pneumophila were investigated with cells exposed to biocide or elevated temperature. Exposure to hypochlorite caused viable nonculturable cells to form. Culturable and viable nonculturable cells showed positive PCR amplification, whereas nonviable cells did not. Viable cells were also specifically detected with mip mRNA as the target, reverse transcription (to form cDNA), and PCR amplification. After exposure to elevated temperature, only viable culturable cells were detected, which corresponded with positive PCR amplification.
研究了使用聚合酶链反应(PCR)和基因探针检测暴露于杀菌剂或高温下的细胞中存活的嗜肺军团菌的方法。暴露于次氯酸盐会导致存活的不可培养细胞形成。可培养和存活的不可培养细胞显示出阳性PCR扩增,而无活力的细胞则没有。还以mip mRNA为靶标,通过逆转录(形成cDNA)和PCR扩增来特异性检测活细胞。暴露于高温后,仅检测到存活的可培养细胞,这与阳性PCR扩增相对应。