分光光度法测定有色溶液中的淀粉酶活性。

Spectrophotometric determination of amylase activity in coloured solutions.

作者信息

Safarík I

机构信息

Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.

出版信息

J Biochem Biophys Methods. 1991 Jan;22(1):61-7. doi: 10.1016/0165-022x(91)90082-8.

DOI:10.1016/0165-022x(91)90082-8

PMID:1706372

Abstract

A simple procedure for spectrophotometric determination of amylase activity in coloured solutions is described. Starch cross-linked with a bifunctional reagent in the presence of black drawing ink is used as an insoluble chromolytic substrate. The absorbances of reaction mixture filtrates are read in the near-infrared region (at 800-900 nm) where the majority of coloured substances does not absorb; on the contrary, black drawing ink released from the substrate during the action of amylase absorbs strongly even at these wavelengths.

摘要

本文描述了一种用于分光光度法测定有色溶液中淀粉酶活性的简单方法。在黑色绘图墨水存在下，与双功能试剂交联的淀粉用作不溶性显色底物。反应混合物滤液的吸光度在近红外区域（800 - 900 nm）读取，在此区域大多数有色物质不吸收；相反，淀粉酶作用过程中从底物释放的黑色绘图墨水即使在这些波长下也有强烈吸收。