Safarík I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
J Biochem Biophys Methods. 1989 Aug-Sep;19(2-3):201-6. doi: 10.1016/0165-022x(89)90026-2.
A simple procedure for spectrophotometric determination of proteolytic activity in coloured solutions is described. Gelatin cross-linked with glutaric dialdehyde or formaldehyde in the presence of black drawing ink is used as an insoluble chromolytic substrate. The absorbances of reaction mixture filtrates are read in the near infra-red region (at 800-900 nm) where the majority of coloured substances does not absorb; on the contrary black drawing ink released from the substrate during the proteolysis absorbs strongly even at these wavelengths.
描述了一种用于分光光度法测定有色溶液中蛋白水解活性的简单方法。在黑色绘图墨水存在下，与戊二醛或甲醛交联的明胶用作不溶性显色底物。反应混合物滤液的吸光度在近红外区域（800 - 900 nm）读取，在此区域大多数有色物质不吸收；相反，蛋白水解过程中从底物释放的黑色绘图墨水即使在这些波长下也有强烈吸收。
