Safarík I, Safaríková M
Department of Analytical Chemistry and Biochemistry, Institute of Landscape Ecology, Ceské Budĕjovice, Czech Republic.
J Biochem Biophys Methods. 1994 Mar;28(2):131-6. doi: 10.1016/0165-022x(94)90027-2.
A new procedure for the determination of effective proteolytic activity in biodetergents has been developed. Effective enzyme activity is defined as the activity exhibited by the tested enzyme under real washing conditions, i.e., in the water suspensions of the biodetergent tested at the working temperature (usually 40 degrees C). Two insoluble chromolytic substrates, namely black gelatin (gelatin cross-linked with glutaraldehyde in the presence of black drawing ink) and the protease substrate based on the immobilization of dyed casein in the structure of polyacrylamide gel (blue casein-PAAG) were successfully used. It was found that there is a great difference in effective proteolytic activity among various biodetergents. The proposed procedure is suitable both for the manufacturers for a quick control of their products and for the quality control laboratories.
已开发出一种测定生物洗涤剂中有效蛋白水解活性的新方法。有效酶活性定义为被测酶在实际洗涤条件下，即在工作温度（通常为40摄氏度）下测试的生物洗涤剂水悬浮液中所表现出的活性。成功使用了两种不溶性显色底物，即黑色明胶（在黑色绘图墨水存在下与戊二醛交联的明胶）和基于将染色酪蛋白固定在聚丙烯酰胺凝胶结构中的蛋白酶底物（蓝色酪蛋白-PAAG）。结果发现，各种生物洗涤剂之间的有效蛋白水解活性存在很大差异。所提出的方法适用于制造商对其产品进行快速控制，也适用于质量控制实验室。
