Ruo S L, Mitchell S W, Kiley M P, Roumillat L F, Fisher-Hoch S P, McCormick J B
Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Gen Virol. 1991 Mar;72 ( Pt 3):549-55. doi: 10.1099/0022-1317-72-3-549.
Monoclonal antibodies (MAbs) were produced against two African arenaviruses, Lassa virus and Mopeia virus. Competitive binding analysis of MAbs identified four antigenic sites on the nucleoprotein (NP), two on glycoprotein 1 (GP1) and six on glycoprotein 2 (GP2) of the Josiah strain of Lassa virus. 64 virus isolates from western, central and southern Africa were all consistently distinguishable by MAbs to certain epitopic sites on GP1, GP2 and NP viral proteins. Furthermore, MAbs to Lassa virus GP1 and NP uniformly distinguished viruses from the West African countries of Sierra Leone, Liberia and Guinea from those of Nigeria. GP2-directed MAbs to two African arenaviruses reacted broadly with South American arenaviruses demonstrating that an epitopic site on GP2 may be the most highly conserved antigen in the arenavirus group.
制备了针对两种非洲沙粒病毒——拉沙病毒和莫佩亚病毒的单克隆抗体。对单克隆抗体的竞争性结合分析确定了拉沙病毒约西亚毒株核蛋白（NP）上的四个抗原位点、糖蛋白1（GP1）上的两个抗原位点以及糖蛋白2（GP2）上的六个抗原位点。来自非洲西部、中部和南部的64株病毒分离株，通过针对病毒蛋白GP1、GP2和NP上某些表位的单克隆抗体，始终能够被区分开来。此外，针对拉沙病毒GP1和NP的单克隆抗体能将来自西非国家塞拉利昂、利比里亚和几内亚的病毒与尼日利亚的病毒明确区分开来。针对两种非洲沙粒病毒的GP2定向单克隆抗体与南美沙粒病毒有广泛反应，这表明GP2上的一个表位可能是沙粒病毒属中最保守的抗原。
