Roeselers Guus, Zippel Barbara, Staal Marc, van Loosdrecht Mark, Muyzer Gerard
Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
FEMS Microbiol Ecol. 2006 Nov;58(2):169-78. doi: 10.1111/j.1574-6941.2006.00172.x.
Phototrophic biofilms were cultivated simultaneously using the same inoculum in three identical flow-lane microcosms located in different laboratories. The growth rates of the biofilms were similar in the different microcosms, but denaturing gradient gel electrophoresis (DGGE) analysis of both 16S and 18S rRNA gene fragments showed that the communities developed differently in terms of species richness and community composition. One microcosm was dominated by Microcoleus and Phormidium species, the second microcosm was dominated by Synechocystis and Phormidium species, and the third microcosm was dominated by Microcoleus- and Planktothrix- affiliated species. No clear effect of light intensity on the cyanobacterial community composition was observed. In addition, DGGE profiles obtained from the cultivated biofilms showed a low resemblance with the profiles derived from the inoculum. These findings demonstrate that validation of reproducibility is essential for the use of microcosm systems in microbial ecology studies.
利用相同接种物在位于不同实验室的三个相同流动通道微宇宙中同时培养光合生物膜。不同微宇宙中生物膜的生长速率相似，但对16S和18S rRNA基因片段进行的变性梯度凝胶电泳（DGGE）分析表明，群落的物种丰富度和群落组成发展不同。一个微宇宙以鞘丝藻属和席藻属物种为主，第二个微宇宙以聚球藻属和席藻属物种为主，第三个微宇宙以与鞘丝藻属和席藻属相关的物种为主。未观察到光照强度对蓝细菌群落组成有明显影响。此外，从培养的生物膜获得的DGGE图谱与接种物的图谱相似度较低。这些发现表明，对于微生物生态学研究中使用微宇宙系统而言，验证可重复性至关重要。
