Hishinuma M, Ohashi K-I, Yamauchi N, Kashima T, Uozaki H, Ota S, Kodama T, Aburatani H, Fukayama M
Department of Pathology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Histopathology. 2006 Nov;49(5):479-86. doi: 10.1111/j.1365-2559.2006.02522.x.
Glypican 3 (GPC3) is a cell surface heparan sulphate proteoglycan expressed specifically in the fetal liver and malignant neoplasms of hepatocyte lineage. The aim was to evaluate the significance of GPC3 in alpha-fetoprotein (AFP)-producing gastric carcinoma (GC) and other forms of GC.
We immunohistochemically evaluated GPC3 expression in representative cases of AFP-producing GC and in a tissue microarray of a consecutive series of GCs with other markers of hepatocyte lineage (AFP, PIVKA-II and hepatocyte antigen, HEP). In a series of 10 cases of AFP-producing GC, we observed immunohistochemical positivity for GPC3, PIVKA-II and HEP in 10, three and three cases in components with a hepatoid pattern and in nine, two and five cases in components with a non-hepatoid pattern, respectively. In a series of 118 cases of GC, we observed positivity for AFP, GPC3, PIVKA-II and HEP in one (0.8%), four (3.4%), six (5.1%) and 26 cases (22%), respectively. GPC3 was observed concurrently with AFP and discordantly with PIVKA-II and HEP. GPC3 positivity was clearly stronger in a larger area compared with immunoreactivity for AFP.
GPC3 is a sensitive marker for AFP-producing GC and its hepatoid component and is therefore useful to identify this aggressive subgroup of GC.
磷脂酰肌醇蛋白聚糖3（GPC3）是一种细胞表面硫酸乙酰肝素蛋白聚糖，在胎儿肝脏和肝细胞谱系的恶性肿瘤中特异性表达。本研究旨在评估GPC3在产生甲胎蛋白（AFP）的胃癌（GC）及其他类型GC中的意义。
我们采用免疫组织化学方法评估了GPC3在产生AFP的GC代表性病例以及一系列连续GC组织芯片中的表达情况，同时检测了其他肝细胞谱系标志物（AFP、异常凝血酶原-II和肝细胞抗原，HEP）。在一组10例产生AFP的GC病例中，我们观察到在具有肝样模式的成分中，GPC3、异常凝血酶原-II和HEP的免疫组化阳性率分别为10例（100%）、3例（30%）和3例（30%）；在具有非肝样模式的成分中，分别为9例（90%）、2例（20%）和5例（50%）。在一组118例GC病例中，我们观察到AFP、GPC3、异常凝血酶原-II和HEP的阳性率分别为1例（0.8%）、4例（3.4%）、6例（5.1%）和26例（22%）。GPC3与AFP同时出现，与异常凝血酶原-II和HEP不一致。与AFP的免疫反应性相比，GPC3阳性在更大面积上明显更强。
GPC3是产生AFP的GC及其肝样成分的敏感标志物，因此有助于识别这一侵袭性GC亚组。
