Rahbari Mohammad, Pecqueux Mathieu, Aust Daniela, Stephan Holger, Tiebel Oliver, Chatzigeorgiou Antonios, Tonn Torsten, Baenke Franziska, Rao Venkatesh, Ziegler Nicole, Greif Helena, Lin Kuailu, Weitz Juergen, Rahbari Nuh Nabi, Kahlert Christoph
Department of Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technical University Dresden, D-01307 Dresden, Germany.
Institute for Pathology, University Hospital Carl Gustav Carus, Technical University Dresden, D-01307 Dresden, Germany.
J Clin Med. 2019 May 16;8(5):696. doi: 10.3390/jcm8050696.
Exosomes are nano-sized membranous vesicles of endosomal origin that carry nucleic acids, lipids and proteins. The cargo of exosomes is cell origin specific and the release of these exosomes and uptake by an acceptor cell is seen as a vital element of cell-cell communication. Here, we sought to investigate the diagnostic and prognostic value of the expression of glypican 3 () on primary gastro-esophageal adenocarcinoma (GEA) tissue () and corresponding serum exosomes (). Circulating exosomes were extracted from serum samples of 49 patients with GEA and 56 controls. Extracted exosomes were subjected to flow cytometry for the expression of and expression on primary GEA tissue samples was determined by immunohistochemistry and correlated to clinicopathological parameters. We found decreased levels in GEA patients compared to healthy controls ( < 0.0001) and high expression. This was significantly associated with poor overall survival (high vs. low : 87.40 vs. 60.93 months, = 0.041, high vs. low : 58.03 vs. 84.70 months, = 0.044). Cox regressional analysis confirmed as an independent prognostic biomarker for GEA ( = 0.02) and expression was validated in two independent cohorts. Our findings demonstrate that and can be used as potential diagnostic and prognostic biomarkers for GEA.
外泌体是源自内体的纳米级膜泡，携带核酸、脂质和蛋白质。外泌体的 cargo 具有细胞起源特异性，这些外泌体的释放以及被受体细胞摄取被视为细胞间通讯的重要元素。在此，我们试图研究磷脂酰肌醇蛋白聚糖 3（）在原发性胃食管腺癌（GEA）组织（）和相应血清外泌体（）上表达的诊断和预后价值。从 49 例 GEA 患者和 56 例对照的血清样本中提取循环外泌体。对提取的外泌体进行流式细胞术检测的表达，通过免疫组织化学测定原发性 GEA 组织样本中的表达，并与临床病理参数相关联。我们发现与健康对照相比，GEA 患者的水平降低（<0.0001）且表达较高。这与较差的总生存期显著相关（高表达组与低表达组：87.40 个月对 60.93 个月，=0.041，高表达组与低表达组：58.03 个月对 84.70 个月，=0.044）。Cox 回归分析证实为 GEA 的独立预后生物标志物（=0.02），且在两个独立队列中验证了表达。我们的研究结果表明，和可作为 GEA 的潜在诊断和预后生物标志物。
