Brocker Melanie, Bott Michael
Institut für Biotechnologie 1, Forschungszentrum Jülich, Jülich, Germany.
FEMS Microbiol Lett. 2006 Nov;264(2):205-12. doi: 10.1111/j.1574-6968.2006.00456.x.
Previous analysis of a Corynebacterium glutamicum Delta mtrAB mutant showed that the MtrAB two-component signal transduction system influences the expression of genes involved in cell wall metabolism or osmoregulation, but it remained unknown whether this influence is direct or indirect. In order to identify the direct target genes of the response regulator MtrA, chromatin immunoprecipitation as a genome-wide approach and DNA affinity chromatography as a gene-specific approach were used. The results indicate that mepA and nlpC, both encoding putative cell wall peptidases, are directly repressed by MtrA, whereas proP and betP, both encoding carriers for compatible solutes, are directly activated by MtrA.
先前对谷氨酸棒杆菌ΔmtrAB突变体的分析表明，MtrAB双组分信号转导系统影响参与细胞壁代谢或渗透调节的基因表达，但这种影响是直接的还是间接的仍不清楚。为了鉴定应答调节因子MtrA的直接靶基因，采用了全基因组范围的染色质免疫沉淀法和基因特异性的DNA亲和色谱法。结果表明，编码假定细胞壁肽酶的mepA和nlpC被MtrA直接抑制，而编码相容性溶质载体的proP和betP被MtrA直接激活。
