Institute of Microbiology and Biotechnology, University of Ulm, 89069, Ulm, Germany.
Institute of Biochemical Engineering, University of Stuttgart, 71032, Stuttgart, Germany.
Appl Microbiol Biotechnol. 2018 Jul;102(14):5901-5910. doi: 10.1007/s00253-018-9085-3. Epub 2018 May 26.
Corynebacterium glutamicum is an industrial workhorse used for the production of amino acids and a variety of other chemicals and fuels. Within its regulatory repertoire, C. glutamicum possesses RamA which was initially identified as essential transcriptional regulator of acetate metabolism. Further studies revealed its relevance for ethanol and propionate catabolism and also identified RamA to function as global regulator in the metabolism of C. glutamicum. Thereby, RamA acts as transcriptional activator or repressor of genes encoding enzymes which are involved in carbon uptake, central carbon metabolism, and cell wall synthesis. RamA controls the expression of target genes either directly and/or indirectly by constituting feed-forward loop type of transcriptional motifs with other regulators such as GlxR, SugR, RamB, and GntR1. In this review, we summarize the current knowledge on RamA, its regulon, and its regulatory interplay with other transcriptional regulators coordinating the metabolism of C. glutamicum.
谷氨酸棒杆菌是一种工业生产用菌，用于生产氨基酸和各种其他化学品和燃料。在其调控机制中，谷氨酸棒杆菌拥有 RamA，最初被鉴定为乙酸代谢的必需转录调控因子。进一步的研究表明，RamA 与乙醇和丙酸的分解代谢有关，并确定 RamA 在谷氨酸棒杆菌的代谢中作为全局调控因子发挥作用。因此，RamA 作为转录激活剂或抑制剂，调节参与碳摄取、中心碳代谢和细胞壁合成的酶编码基因的表达。RamA 通过与其他转录因子（如 GlxR、SugR、RamB 和 GntR1）构成正反馈环类型的转录基序，直接和/或间接地控制靶基因的表达。在这篇综述中，我们总结了 RamA 的最新知识，包括其调控组和与其他转录因子的调控相互作用，以协调谷氨酸棒杆菌的代谢。
