Möker Nina, Krämer Jens, Unden Gottfried, Krämer Reinhard, Morbach Susanne
Institut für Biochemie der Universität zu Köln, Zülpicher Str. 47, D-50674 Köln, Germany.
J Bacteriol. 2007 May;189(9):3645-9. doi: 10.1128/JB.01920-06. Epub 2007 Feb 9.
The two-component system MtrBA is involved in the osmostress response of Corynebacterium glutamicum. MtrB was reconstituted in a functionally active form in liposomes and showed autophosphorylation and phosphatase activity. In proteoliposomes, MtrB activity was stimulated by monovalent cations used by many osmosensors for the detection of hypertonicity. Although MtrB was activated by monovalent cations, they lead in vitro to a general stabilization of histidine kinases and do not represent the stimulus for MtrB to sense hyperosmotic stress.
双组分系统MtrBA参与谷氨酸棒杆菌的渗透应激反应。MtrB在脂质体中以功能活性形式重构,并表现出自磷酸化和磷酸酶活性。在蛋白脂质体中,MtrB的活性受到许多渗透传感器用于检测高渗的单价阳离子的刺激。尽管MtrB被单价阳离子激活,但它们在体外导致组氨酸激酶的普遍稳定,并不代表MtrB感知高渗应激的刺激因素。